Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,336	G→A	A107T (GCC→ACC)	arnD →	undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,336	0	G	A	100.0%	170.3 / NA	53	A107T (GCC→ACC)	arnD	undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/27); total (26/27)

CGCTCAAACGCGGCATCGCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACGCCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCAACCGGTAACCTGTTCTGCCGCTGC  >  NC_000913/2370204‑2370466

cgcTCAAACGCGGCATCGCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTggg                                                                                                                              <  1:351824/139‑1 (MQ=255)

  cTCAAACGCGGCATCGCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGAt                                                                                                                            >  2:1057361/1‑139 (MQ=255)

     aaaCGCGGCATCGCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCAc                                                                                                                         <  2:1492746/139‑1 (MQ=255)

          cgGCATCGCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGc                                                                                                                    >  2:824904/1‑139 (MQ=255)

              acagCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCAAGGTAAAGAGATTGGTCATGCCAATGCCGATAACATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTgg                                                                                                                <  2:339571/136‑1 (MQ=255)

                      aTGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCg                                                                                                        <  1:824904/139‑1 (MQ=255)

                        ggCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTa                                                                                                      <  1:1462369/139‑1 (MQ=255)

                         gCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAg                                                                                                     <  1:1057361/139‑1 (MQ=255)

                         gCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAg                                                                                                     <  2:979922/139‑1 (MQ=255)

                          cTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGc                                                                                                    >  1:5178/1‑139 (MQ=255)

                           tGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGTAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCg                                                                                                   <  1:1140629/139‑1 (MQ=255)

                           tGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCg                                                                                                   <  2:5178/139‑1 (MQ=255)

                             ggATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGTCGATATCATTCGCGAAGCGGATAAACATCAAGGAGTTGGCCTGCACACCTGGCATCACCATGCCTGTCAATCCCGTAGCTGt                                                                                                 >  1:339571/1‑139 (MQ=255)

                                         ggCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         <  2:796284/135‑1 (MQ=255)

                                         ggCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         >  1:796284/1‑135 (MQ=255)

                                              gTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAAc                                                                                >  2:1147944/1‑139 (MQ=255)

                                                    ccTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         >  1:184213/1‑124 (MQ=255)

                                                    ccTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         <  2:184213/124‑1 (MQ=255)

                                                      tGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATg                                                                                                                     <  1:642473/94‑1 (MQ=255)

                                                      tGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATg                                                                                                                     >  2:642473/1‑94 (MQ=255)

                                                        gCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCaaa                                                                                 >  2:599311/1‑128 (MQ=255)

                                                        gCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCaaa                                                                                 <  1:599311/128‑1 (MQ=255)

                                                         ccAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTgg                                                                                                                >  2:127644/1‑96 (MQ=255)

                                                         ccAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTgg                                                                                                                <  1:127644/96‑1 (MQ=255)

                                                         ccAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGc                                                                                                    <  2:528627/108‑1 (MQ=255)

                                                         ccAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGc                                                                                                    >  1:528627/1‑108 (MQ=255)

                                                         ccAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATcgacg                                                                     >  2:1309424/1‑139 (MQ=255)

                                                              tAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         <  2:148604/114‑1 (MQ=255)

                                                              tAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         >  1:148604/1‑114 (MQ=255)

                                                                      ttGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCAc                                                                                                                         >  2:299391/1‑74 (MQ=255)

                                                                      ttGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCAc                                                                                                                         <  1:299391/74‑1 (MQ=255)

                                                                       tGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGa                                                                                                                             <  2:351341/69‑1 (MQ=255)

                                                                       tGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGa                                                                                                                             >  1:351341/1‑69 (MQ=255)

                                                                       tGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCg                                                                                                        <  1:569926/90‑1 (MQ=255)

                                                                       tGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCg                                                                                                        >  2:569926/1‑90 (MQ=255)

                                                                            aTGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACAcc                                                                                                                                  >  2:1260080/1‑59 (MQ=255)

                                                                            aTGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACAcc                                                                                                                                  <  1:1260080/59‑1 (MQ=255)

                                                                            aTGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCg                                                  <  1:1068749/139‑1 (MQ=255)

                                                                             tGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGc                                                 >  2:1362303/1‑139 (MQ=255)

                                                                                        atatCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTgg                                                                                           <  2:1425013/86‑1 (MQ=255)

                                                                                        atatCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTgg                                                                                           >  1:1425013/1‑86 (MQ=255)

                                                                                                cGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         <  1:844089/80‑1 (MQ=255)

                                                                                                cGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGa                                                                                         >  2:844089/1‑80 (MQ=255)

                                                                                                   gAAGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTc                           <  1:1309424/139‑1 (MQ=255)

                                                                                                     aGCGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCaa                         >  1:310754/1‑139 (MQ=255)

                                                                                                       cGGCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCAAcc                       >  2:910835/1‑139 (MQ=255)

                                                                                                         gCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCAcgc                                                          <  2:247665/102‑1 (MQ=255)

                                                                                                         gCTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCAcgc                                                          >  1:247665/1‑102 (MQ=255)

                                                                                                          cTAAACATCACGAAGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCAACCGGt                    <  1:1147944/139‑1 (MQ=255)

                                                                                                                       aGTCGGCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCAACCGGTAACCTGTTCTGcc       >  1:567613/1‑139 (MQ=255)

                                                                                                                            gCCTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCAACCGGTAACCTGTTCTGCCGCTGc  >  2:353975/1‑139 (MQ=255)

                                                                                                                             ccTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATcga                                                                       <  1:580960/69‑1 (MQ=255)

                                                                                                                             ccTGCACACCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATcga                                                                       >  2:580960/1‑69 (MQ=255)

CGCTCAAACGCGGCATCGCTTTATGGCTGGGATATTTTACTGGCAGGTACGGCCTGGCCAGGTAAAGAGATTGGTCATGCCAATGCCGATATCATTCGTGAAGCGGCTAAACATCACGAAGTCGGCCTGCACGCCTGGGATCACCATGCCTGGCAAGCCCGTAGCGGTAACTGGGATCGGCAAACAATGATCGACGATATTGCACGCGGTCTTCGCACTCTGGAAGAGATTATCGGTCAACCGGTAACCTGTTCTGCCGCTGC  >  NC_000913/2370204‑2370466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A