Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A4 F1 I2 R1 | 780 | 68.3 | 3343050 | 93.5% | 3125751 | 102.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,401,654 | G→A | C133C (TGC→TGT) | nuoE ← | NADH:ubiquinone oxidoreductase, chain E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,401,654 | 0 | G | A | 100.0% | 219.8 / NA | 68 | C133C (TGC→TGT) | nuoE | NADH:ubiquinone oxidoreductase, chain E |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/35); total (33/35) | |||||||||||
AGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAA > NC_000913/2401518‑2401780 | aGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGaca > 1:424876/1‑139 (MQ=255) gATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCaa < 1:343362/139‑1 (MQ=255) tACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTa > 1:591360/1‑139 (MQ=255) cTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCt > 1:805365/1‑139 (MQ=255) gTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGAt > 2:806401/1‑139 (MQ=255) cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcagca < 2:1266754/96‑1 (MQ=255) cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcagca > 1:1266754/1‑96 (MQ=255) cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTg > 2:689062/1‑129 (MQ=255) cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTg < 1:689062/129‑1 (MQ=255) cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGtt > 2:921974/1‑139 (MQ=255) gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCgg > 1:1165134/1‑99 (MQ=255) gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCgg < 2:1165134/99‑1 (MQ=255) gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCtttt > 2:945119/1‑139 (MQ=255) gggTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAg < 1:806401/139‑1 (MQ=255) aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcag > 1:1032317/1‑74 (MQ=255) aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcag < 2:1032317/74‑1 (MQ=255) aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc > 2:928621/1‑106 (MQ=255) aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc < 1:928621/106‑1 (MQ=255) aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg < 1:921974/139‑1 (MQ=255) aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg < 1:1308933/139‑1 (MQ=255) cgcgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGa < 2:424876/139‑1 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTgg > 1:686533/1‑64 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTgg < 2:686533/64‑1 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc > 2:1316811/1‑99 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc < 1:1316811/99‑1 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt > 1:479172/1‑139 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt < 2:591360/139‑1 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt > 1:1561403/1‑139 (MQ=255) gTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACGGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATa < 2:749782/139‑1 (MQ=255) tCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGACAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAc > 2:1636941/1‑139 (MQ=255) aTCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCg > 2:1515468/1‑139 (MQ=255) gATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGACAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTg < 1:1636941/139‑1 (MQ=255) tcatGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc < 1:1099504/109‑1 (MQ=255) tcatGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc > 2:1099504/1‑109 (MQ=255) gTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa > 1:1082153/1‑113 (MQ=255) gTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa < 2:1082153/113‑1 (MQ=255) tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt < 2:1383019/125‑1 (MQ=255) tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt > 1:1383019/1‑125 (MQ=255) gCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCAc > 2:269101/1‑139 (MQ=255) cccTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACg < 1:945119/139‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc < 1:575290/64‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtct < 1:71976/88‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtct > 2:71976/1‑88 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc > 2:211993/1‑107 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:211993/107‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 1:27592/116‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc > 2:27592/1‑116 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGt < 2:1561403/139‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGt < 1:269101/139‑1 (MQ=255) ttATCACAGTTCCCCAGACAGCAAGATGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc > 2:575290/1‑64 (MQ=255) tATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 2:928382/106‑1 (MQ=255) tATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc > 1:928382/1‑106 (MQ=255) acaGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACaa < 2:805365/139‑1 (MQ=255) aGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa > 2:1632932/1‑96 (MQ=255) aGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa < 1:1632932/96‑1 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:1001553/98‑1 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc > 2:1001553/1‑98 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCTTCAAATGTCGATTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg > 2:1064406/1‑86 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc > 1:1071322/1‑53 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc < 2:1071322/53‑1 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc < 1:190415/84‑1 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc > 2:190415/1‑84 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg < 1:1064406/86‑1 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAc < 1:1484015/95‑1 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAc > 2:1484015/1‑95 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGc > 2:429134/1‑125 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGc < 1:429134/125‑1 (MQ=255) cccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 2:209584/104‑1 (MQ=255) cccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc > 1:209584/1‑104 (MQ=255) | AGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAA > NC_000913/2401518‑2401780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |