Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,439,222 |
G→A |
P300L (CCA→CTA) |
yfcJ ← |
putative arabinose efflux transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,439,222 | 0 | G | A | 100.0%
| 87.9
/ NA
| 29 | P300L (CCA→CTA) | yfcJ | putative arabinose efflux transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/14); total (15/14) |
CGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCA > NC_000913/2439088‑2439347
|
cggcgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGc > 2:1246842/1‑139 (MQ=255)
cgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTg > 1:40542/1‑139 (MQ=255)
gCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGcaaca < 1:1246842/139‑1 (MQ=255)
cgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCg > 2:580114/1‑139 (MQ=255)
cgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCg < 2:574335/139‑1 (MQ=255)
ccACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGcc < 1:50921/103‑1 (MQ=255)
ccACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGcc > 2:50921/1‑103 (MQ=255)
gCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAg < 1:1591615/114‑1 (MQ=255)
gCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAg > 2:1591615/1‑114 (MQ=255)
aGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGc > 1:50710/1‑78 (MQ=255)
aGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGc < 2:50710/78‑1 (MQ=255)
cgcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGcc < 2:536864/77‑1 (MQ=255)
cgcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGcc > 1:536864/1‑77 (MQ=255)
gcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCaa > 2:872210/1‑139 (MQ=255)
gcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCaa < 1:872210/139‑1 (MQ=255)
ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCa > 2:1173363/1‑74 (MQ=255)
ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCa < 1:1173363/74‑1 (MQ=255)
cGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGcaa < 1:618436/72‑1 (MQ=255)
cGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGcaa > 2:618436/1‑72 (MQ=255)
cGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATc < 2:301710/139‑1 (MQ=255)
tCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGTAACAAGCCCa > 2:266435/1‑68 (MQ=255)
tCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGTAACAAGCCCa < 1:266435/68‑1 (MQ=255)
gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACa > 2:419432/1‑75 (MQ=255)
gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACa < 1:419432/75‑1 (MQ=255)
cgcgCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCagagag > 2:1291896/1‑76 (MQ=255)
cgcgCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCagagag < 1:1291896/76‑1 (MQ=255)
cgcgCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACg > 1:257093/1‑139 (MQ=255)
cgcCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGAc > 2:955778/1‑139 (MQ=255)
gACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCTCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCTCGACAAATGCGCCGCCa < 1:955778/139‑1 (MQ=255)
|
CGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCA > NC_000913/2439088‑2439347
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
