Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,441,742	G→A	intergenic (‑137/‑22)	fabB ← / → mnmC	3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,441,742	0	G	A	100.0%	246.8 / NA	76	intergenic (‑137/‑22)	fabB/mnmC	3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (43/33); total (43/33)

TACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAG  >  NC_000913/2441610‑2441872

tACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTa                                                                                                                              >  2:501546/1‑139 (MQ=255)

tACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTa                                                                                                                              >  1:1825366/1‑139 (MQ=255)

 aCCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTaa                                                                                                                             <  2:1044330/139‑1 (MQ=255)

   ctctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGa                                                                                                                           >  2:1056897/1‑139 (MQ=255)

   ctctGTAAGTCCCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGa                                                                                                                           <  2:1187673/139‑1 (MQ=255)

        tAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGt                                                                                                                      <  2:99069/139‑1 (MQ=255)

          aGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTaa                                                                                                                    <  1:1652345/139‑1 (MQ=255)

          aGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTaa                                                                                                                    <  2:801727/139‑1 (MQ=255)

             cGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGAc                                                                                                                          >  1:207427/1‑130 (MQ=255)

             cGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGAc                                                                                                                          <  2:207427/130‑1 (MQ=255)

              gCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTa                                                                                                                >  2:1066000/1‑139 (MQ=255)

               cacaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAAc                                                                                                                   >  2:1468328/1‑135 (MQ=255)

               cacaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAAc                                                                                                                   <  1:1468328/135‑1 (MQ=255)

                acaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACg                                                                                                              >  1:1711392/1‑139 (MQ=255)

                  aTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg                                                                                                            <  2:362147/139‑1 (MQ=255)

                          aGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  2:1825366/139‑1 (MQ=255)

                              tCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACg                                                                                                              >  2:21789/1‑125 (MQ=255)

                              tCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACg                                                                                                              <  1:21789/125‑1 (MQ=255)

                              tCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTcc                                                                                                >  2:432007/1‑139 (MQ=255)

                                          tAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGcc                                                                                    <  1:501546/139‑1 (MQ=255)

                                               tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCaccacc                                                                                                                                  <  1:1216794/88‑1 (MQ=255)

                                               tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCt                                                                               >  2:651439/1‑139 (MQ=255)

                                                    ttGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg                                                                                                            <  2:268290/105‑1 (MQ=255)

                                                    ttGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg                                                                                                            >  1:268290/1‑105 (MQ=255)

                                                       tACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTa                                                                       >  2:29557/1‑139 (MQ=255)

                                                       tACGCCGAACAAGTCCGATCAACCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAGCCTCGAGTTTa                                                                       <  1:1066000/139‑1 (MQ=255)

                                                            cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:432007/139‑1 (MQ=255)

                                                            cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:1056897/139‑1 (MQ=255)

                                                            cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:29557/139‑1 (MQ=255)

                                                              aaCAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGa                                                                >  1:1178491/1‑139 (MQ=255)

                                                                   gTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTa                                                           >  2:1712078/1‑139 (MQ=255)

                                                                        aTCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCt                                                      >  1:1513909/1‑139 (MQ=255)

                                                                              cATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  2:49019/1‑87 (MQ=255)

                                                                              cATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  1:49019/87‑1 (MQ=255)

                                                                                tttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:1518604/1‑119 (MQ=255)

                                                                                tttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:1518604/119‑1 (MQ=255)

                                                                                  tAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCgag                                            >  1:1527671/1‑139 (MQ=255)

                                                                                       gagaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGAtttt                                       >  1:1445999/1‑139 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAaca                                                                                                       >  2:311838/1‑72 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAaca                                                                                                       <  1:311838/72‑1 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac                                                                                                   >  2:1506562/1‑76 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac                                                                                                   <  1:1506562/76‑1 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:664703/1‑109 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:664703/109‑1 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGAc                                    >  2:890055/1‑139 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGAc                                    >  1:1435650/1‑139 (MQ=255)

                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGAc                                    >  1:1141661/1‑139 (MQ=255)

                                                                                             tttGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACg                                   >  2:650153/1‑137 (MQ=255)

                                                                                             tttGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACg                                   <  1:650153/137‑1 (MQ=255)

                                                                                                   cAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCTTACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTAt                           <  2:1141661/139‑1 (MQ=255)

                                                                                                           cacacaTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTc                      >  1:1742340/1‑136 (MQ=255)

                                                                                                           cacacaTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTc                      <  2:1742340/136‑1 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  2:361858/1‑53 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  1:361858/53‑1 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:1457252/87‑1 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  2:1457252/1‑87 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  2:903949/1‑139 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  1:1407617/1‑139 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  1:350729/1‑139 (MQ=255)

                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  2:452069/1‑139 (MQ=255)

                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:227864/1‑85 (MQ=255)

                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:345010/1‑85 (MQ=255)

                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:345010/85‑1 (MQ=255)

                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:227864/85‑1 (MQ=255)

                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATTTCTATTTTTCCAACGATaa             <  2:1223433/138‑1 (MQ=255)

                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATaa             >  1:1223433/1‑138 (MQ=255)

                                                                                                                    cTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGc                                                                                     <  2:33067/64‑1 (MQ=255)

                                                                                                                    cTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGc                                                                                     >  1:33067/1‑64 (MQ=255)

                                                                                                                          tCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATaa             >  2:346812/1‑130 (MQ=255)

                                                                                                                          tCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATaa             <  1:346812/130‑1 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  1:1595055/1‑41 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  2:1595055/41‑1 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  1:1440278/41‑1 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  2:1440278/1‑41 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAag  >  2:291999/1‑139 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAag  >  1:612399/1‑139 (MQ=255)

                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAag  >  2:96947/1‑139 (MQ=255)

TACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAG  >  NC_000913/2441610‑2441872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A