Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,441,742 |
G→A |
intergenic (‑137/‑22) |
fabB ← / → mnmC |
3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,441,742 | 0 | G | A | 94.7%
| 110.5
/ ‑0.4
| 38 | intergenic (‑137/‑22) | fabB/mnmC | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/15); minor base . (1/1); total (22/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 |
CCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAG > NC_000913/2441612‑2441874
|
ccTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAg < 2:1177014/139‑1 (MQ=255)
ctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACg > 1:1093436/1‑139 (MQ=255)
tAAGTCGCACATAGAGTAAGTTTCGAATGCATAATAGCGTGCACTGGTACGGTGAACAAGTCCGATCAGCCACTTAATAGAGAAATTTGCGCAGCGTTACACACATCGCTAAGAGCGATCCACCACCTGTAAGACGAGt > 1:1100906/1‑139 (MQ=255)
tAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGt > 2:794850/1‑139 (MQ=255)
aTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTCACTTACgcg > 1:805954/1‑137 (MQ=255)
aTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg < 2:96490/139‑1 (MQ=255)
tAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGa < 2:1093436/139‑1 (MQ=255)
gagTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGaaa < 1:510182/139‑1 (MQ=255)
gAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac > 2:739966/1‑134 (MQ=255)
gAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac < 1:739966/134‑1 (MQ=255)
gAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCAt > 2:1339521/1‑139 (MQ=255)
tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCt > 2:1066948/1‑139 (MQ=255)
gTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta > 2:1274579/1‑111 (MQ=255)
gTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta < 1:1274579/111‑1 (MQ=255)
gTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAttt > 2:625911/1‑139 (MQ=255)
cGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCA‑‑‑CCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt > 2:192224/1‑139 (MQ=255)
cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt < 1:775297/139‑1 (MQ=255)
aCAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCA‑‑‑CCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGt < 1:192224/139‑1 (MQ=255)
cGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCa > 2:566625/1‑112 (MQ=255)
cGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCa < 1:566625/112‑1 (MQ=255)
cAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGt > 1:436795/1‑139 (MQ=255)
aaTAGAGAAATTTGCGCAGCCCTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTTCGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCCGTTTcctggga < 1:794850/139‑6 (MQ=255)
gagaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCGGAATtttt > 1:1329194/1‑139 (MQ=255)
aTCGCTAATATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta < 1:966507/53‑1 (MQ=255)
aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta > 2:966507/1‑53 (MQ=255)
aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa > 2:200721/1‑139 (MQ=255)
aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa > 1:314567/1‑139 (MQ=255)
aTCGCTAAGATCGAGCCACCACCTGTAAAACGAGAAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa > 1:885767/1‑139 (MQ=255)
cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATg < 2:1132503/119‑1 (MQ=255)
cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATg > 1:1132503/1‑119 (MQ=255)
aaGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGc < 2:436795/139‑1 (MQ=255)
tCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGa > 1:382724/1‑139 (MQ=255)
aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGa > 1:1241720/1‑64 (MQ=255)
aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGa < 2:1241720/64‑1 (MQ=255)
aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga > 1:1081122/1‑139 (MQ=255)
aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga > 1:244766/1‑139 (MQ=255)
aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga < 1:1339521/139‑1 (MQ=255)
gCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAagag < 1:200721/139‑1 (MQ=255)
|
CCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAG > NC_000913/2441612‑2441874
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
