Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,980,963 |
G→A |
S407L (TCG→TTG) |
araE ← |
arabinose transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,980,963 | 0 | G | A | 100.0%
| 107.0
/ NA
| 34 | S407L (TCG→TTG) | araE | arabinose transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/17); total (17/17) |
GACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATAC > NC_000913/2980827‑2981080
|
gACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAac > 1:529051/1‑139 (MQ=255)
cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAacac < 2:1197291/139‑1 (MQ=255)
ttGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGt < 1:1538015/139‑1 (MQ=255)
cccACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAAt < 2:529051/139‑1 (MQ=255)
cccACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAAt > 1:40856/1‑139 (MQ=255)
aGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAAtt > 1:729504/1‑139 (MQ=255)
agCCAGAACGTACTGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACAcc < 2:303709/75‑1 (MQ=255)
agCCAGAACGTACTGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACAcc > 1:303709/1‑75 (MQ=255)
gAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAgg > 1:660789/1‑139 (MQ=255)
aaCGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCGGTTCGtggtgg < 2:879192/82‑1 (MQ=255)
aaCGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGtggtgg > 1:879192/1‑82 (MQ=255)
cGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATc < 2:660789/139‑1 (MQ=255)
aCCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCcac > 1:624108/1‑139 (MQ=255)
aCCGGCAGCGCCAAGGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCACGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCcac > 2:1017018/1‑139 (MQ=255)
ccAATGCTTTCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcg < 1:1017018/139‑1 (MQ=255)
aTCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCa < 1:1147375/106‑1 (MQ=255)
aTCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCa > 2:1147375/1‑106 (MQ=255)
tCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGca < 2:1500927/110‑1 (MQ=255)
tCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGca > 1:1500927/1‑110 (MQ=255)
tgtCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATaa > 2:500281/1‑139 (MQ=255)
gtCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg > 1:144427/1‑86 (MQ=255)
gtCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg < 2:144427/86‑1 (MQ=255)
aGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg < 1:500281/139‑1 (MQ=255)
aGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg < 2:40856/139‑1 (MQ=255)
aGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAAt < 2:624108/139‑1 (MQ=255)
gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATcg > 2:226106/1‑63 (MQ=255)
gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATcg < 1:226106/63‑1 (MQ=255)
gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg > 2:844658/1‑77 (MQ=255)
gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg < 1:844658/77‑1 (MQ=255)
gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATac > 2:1457175/1‑139 (MQ=255)
cgcgCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCaga < 2:1195513/87‑1 (MQ=255)
cgcgCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCaga > 1:1195513/1‑87 (MQ=255)
atTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg < 1:685507/116‑1 (MQ=255)
atTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg > 2:685507/1‑116 (MQ=255)
|
GACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATAC > NC_000913/2980827‑2981080
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
