Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 775,120 G→A A123A (GCG→GCA)  ybgC → acyl‑CoA thioester hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,1200GA100.0% 239.3 / NA 73A123A (GCG→GCA) ybgCacyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/39);  total (34/39)

ACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCA  >  NC_000913/774985‑775252
                                                                                                                                       |                                                                                                                                    
aCAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACtt                                                                                                                                   <  2:496379/139‑1 (MQ=255)
  aGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                 <  2:877043/139‑1 (MQ=255)
     cTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCaa                                                                                                                              >  1:528756/1‑139 (MQ=255)
                 cAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  <  1:1012713/139‑1 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg                                                                                                                 >  1:107215/1‑139 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg                                                                                                                 >  1:723150/1‑139 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg                                                                                                                 >  1:613331/1‑139 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg                                                                                                                 >  1:552185/1‑139 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  >  1:213286/1‑138 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  <  2:213286/138‑1 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcg                                                                                                                   >  2:48362/1‑137 (MQ=255)
                  aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcg                                                                                                                   <  1:48362/137‑1 (MQ=255)
                           cACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAg                                                                                                        <  1:1044694/139‑1 (MQ=255)
                            aCCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGc                                                                                                       <  2:6977/139‑1 (MQ=255)
                                ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGACTGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  <  1:647338/124‑1 (MQ=255)
                                ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGTGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtg                                                                                                   >  2:523178/1‑139 (MQ=255)
                                ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  >  2:647338/1‑124 (MQ=255)
                                 tttGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                  <  2:1045454/139‑1 (MQ=255)
                                     gTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                              <  2:875915/139‑1 (MQ=255)
                                        ttCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                >  2:193035/1‑102 (MQ=255)
                                        ttCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                <  1:193035/102‑1 (MQ=255)
                                           aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGTGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                        <  1:523178/139‑1 (MQ=255)
                                           aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                        >  2:55892/1‑139 (MQ=255)
                                           aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                        <  2:552185/139‑1 (MQ=255)
                                           aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                        >  2:908316/1‑139 (MQ=255)
                                                       gTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAttt                                                                            <  1:224805/139‑1 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                >  2:620719/1‑83 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                <  1:620719/83‑1 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCTAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                        >  1:571014/1‑123 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                              <  1:741226/117‑1 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                              >  2:741226/1‑117 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACAt                                                                                           <  2:551536/120‑1 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACAt                                                                                           >  1:551536/1‑120 (MQ=255)
                                                           aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                        <  2:571014/123‑1 (MQ=255)
                                                            cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  >  1:547065/1‑96 (MQ=255)
                                                            cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa                                                                                         <  2:778383/121‑1 (MQ=255)
                                                            cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa                                                                                         >  1:778383/1‑121 (MQ=255)
                                                            cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTCGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  <  2:547065/96‑1 (MQ=255)
                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                <  1:364528/81‑1 (MQ=255)
                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                >  2:364528/1‑81 (MQ=255)
                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                 >  1:204462/1‑80 (MQ=255)
                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                 <  2:204462/80‑1 (MQ=255)
                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  <  2:447867/95‑1 (MQ=255)
                                                             gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  >  1:447867/1‑95 (MQ=255)
                                                                   aaTACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGc                                                                <  1:55892/139‑1 (MQ=255)
                                                                   aaTACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGc                                                                <  1:377212/139‑1 (MQ=255)
                                                                        tttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGcc                                                           >  2:18454/1‑139 (MQ=255)
                                                                         ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                          >  1:1035524/1‑139 (MQ=255)
                                                                          tGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCtt                                                         <  2:843926/139‑1 (MQ=255)
                                                                          tGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCtt                                                         <  1:908316/139‑1 (MQ=255)
                                                                             tgaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                  >  2:142350/1‑95 (MQ=255)
                                                                             tgaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                  <  1:142350/95‑1 (MQ=255)
                                                                                    aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                >  2:940199/1‑74 (MQ=255)
                                                                                    aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                <  1:940199/74‑1 (MQ=255)
                                                                                    aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAAc                                               >  1:730514/1‑139 (MQ=255)
                                                                                         agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                  <  2:837250/83‑1 (MQ=255)
                                                                                         agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                  >  1:837250/1‑83 (MQ=255)
                                                                                              cTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  >  2:908112/1‑62 (MQ=255)
                                                                                              cTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                  <  1:908112/62‑1 (MQ=255)
                                                                                                       tGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGt                                                    >  1:126344/1‑115 (MQ=255)
                                                                                                       tGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGt                                                    <  2:126344/115‑1 (MQ=255)
                                                                                                           ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                <  1:479283/51‑1 (MQ=255)
                                                                                                           ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCgg                                                                                                                >  2:479283/1‑51 (MQ=255)
                                                                                                                   cTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                          >  1:3805/1‑97 (MQ=255)
                                                                                                                   cTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                          <  2:3805/97‑1 (MQ=255)
                                                                                                                   cTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAAt                >  1:239147/1‑139 (MQ=255)
                                                                                                                            aaGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTg       <  2:239147/139‑1 (MQ=255)
                                                                                                                              gCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTggg     >  2:318719/1‑139 (MQ=255)
                                                                                                                               ccTCGTGCACTTCCCAAGTCTATTGTCTCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc    <  1:178289/139‑1 (MQ=255)
                                                                                                                               ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc    <  2:723150/139‑1 (MQ=255)
                                                                                                                                 tCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCa  <  2:730514/139‑1 (MQ=255)
                                                                                                                                    tGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                              >  1:9279/1‑60 (MQ=255)
                                                                                                                                    tGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                              <  2:9279/60‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
ACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCA  >  NC_000913/774985‑775252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A