Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 760 52.7 2744488 90.2% 2475528 105.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 775,120 G→A A123A (GCG→GCA)  ybgC → acyl‑CoA thioester hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,1200GA100.0% 177.9 / NA 55A123A (GCG→GCA) ybgCacyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/25);  total (30/25)

AGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCAT  >  NC_000913/774987‑775253
                                                                                                                                     |                                                                                                                                     
tataagaggcacgaTCAATGGCTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                  <  1:1348474/126‑1 (MQ=255)
      aaaTAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTc                                                                                                                            >  1:691546/1‑139 (MQ=255)
               cAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                   <  1:1078866/139‑1 (MQ=255)
                 aTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCaa                                                                                                                               <  1:24446/125‑1 (MQ=255)
                 aTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCaa                                                                                                                               >  2:24446/1‑125 (MQ=255)
                       ggCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCTTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTa                                                                                                           >  2:896952/1‑139 (MQ=255)
                       ggCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTa                                                                                                           <  2:348669/139‑1 (MQ=255)
                       ggCACCTCTTGGGTTTTCACGAAACGTATTGCCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTTTGGTTGTTTGCGTTTACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGttgtctcttata                                                                                                           >  2:1348474/1‑128 (MQ=255)
                              ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtg                                                                                                    >  2:546512/1‑139 (MQ=255)
                               tttGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                   <  2:691546/139‑1 (MQ=255)
                                   gTTTTCACGCAACGTATTGTCAACGCCGATAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCAACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTTTTGTCGAGGAGTTTAAGCATtgactga                                                                                               >  1:528762/1‑139 (MQ=255)
                                      ttCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                   <  1:762535/132‑1 (MQ=255)
                                      ttCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                   >  2:762535/1‑132 (MQ=255)
                                       tCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATg                                                                                           >  2:1123168/1‑139 (MQ=255)
                                       tCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATg                                                                                           >  1:445588/1‑139 (MQ=255)
                                        cACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa                                                                                          >  1:545395/1‑139 (MQ=255)
                                         aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                         <  2:954528/139‑1 (MQ=255)
                                           gCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAata                                                                                       <  2:1165999/139‑1 (MQ=255)
                                             aaCGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                         <  2:1042304/135‑1 (MQ=255)
                                             aaCGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                         >  1:1042304/1‑135 (MQ=255)
                                                       cAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                   <  1:1002206/115‑1 (MQ=255)
                                                       cAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                   >  2:1002206/1‑115 (MQ=255)
                                                         aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                   <  1:26016/97‑1 (MQ=255)
                                                         aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                   >  2:26016/1‑97 (MQ=255)
                                                               agaATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAg                                                                   >  2:107432/1‑139 (MQ=255)
                                                                   tACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTa                                                               <  1:107432/139‑1 (MQ=255)
                                                                       ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                           <  1:1123168/139‑1 (MQ=255)
                                                                             aatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                 >  1:1363496/1‑63 (MQ=255)
                                                                             aatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                 <  2:1363496/63‑1 (MQ=255)
                                                                                    cAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCAAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACtt                                              >  2:712063/1‑139 (MQ=255)
                                                                                      gagGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTAt                                            <  2:545395/139‑1 (MQ=255)
                                                                                      gagCTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCACGTGCACTTCCCAAGTCTATTGCCGCGGGGTTTAAGCATTGACTGACATGAATAGCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTAt                                            <  2:528762/139‑1 (MQ=255)
                                                                                       agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTa                                                   >  1:524814/1‑131 (MQ=255)
                                                                                       agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTa                                                   <  2:524814/131‑1 (MQ=255)
                                                                                       agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATc                                           >  1:181427/1‑139 (MQ=255)
                                                                                           tCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                  <  1:931348/48‑1 (MQ=255)
                                                                                           tCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTcc                                                                                                                                  >  2:931348/1‑48 (MQ=255)
                                                                                              ggTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttga                                    >  2:1327598/1‑139 (MQ=255)
                                                                                                   tttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                 >  2:1297053/1‑41 (MQ=255)
                                                                                                   tttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc                                                                                                                                 <  1:1297053/41‑1 (MQ=255)
                                                                                                    ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                   >  2:291238/1‑54 (MQ=255)
                                                                                                    ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgc                                                                                                                   <  1:291238/54‑1 (MQ=255)
                                                                                                         ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGt                         >  2:984639/1‑139 (MQ=255)
                                                                                                                 cTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGc                                                                                                        <  2:1295796/52‑1 (MQ=255)
                                                                                                                 cTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGc                                                                                                        >  1:1295796/1‑52 (MQ=255)
                                                                                                                      aaTGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCAt            >  1:93316/1‑139 (MQ=255)
                                                                                                                           aGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTgg       >  1:149735/1‑139 (MQ=255)
                                                                                                                            gCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAata                                                                                       >  2:157740/1‑58 (MQ=255)
                                                                                                                            gCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAata                                                                                       <  1:157740/58‑1 (MQ=255)
                                                                                                                            gCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTggg      >  2:799168/1‑139 (MQ=255)
                                                                                                                             ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc     <  1:984639/139‑1 (MQ=255)
                                                                                                                             ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc     <  1:546512/139‑1 (MQ=255)
                                                                                                                             ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc     <  2:93316/139‑1 (MQ=255)
                                                                                                                                cGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCat  >  1:1018503/1‑139 (MQ=255)
                                                                                                                                cGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTAGTTTTTCAATCGCATCTTGGGTCat  >  2:882445/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCAT  >  NC_000913/774987‑775253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A