Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
927,205 |
A→G |
R76R (CGT→CGC) |
aat ← |
leucyl/phenylalanyl‑tRNA‑protein transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 927,205 | 0 | A | G | 100.0%
| 157.1
/ NA
| 46 | R76R (CGT→CGC) | aat | leucyl/phenylalanyl‑tRNA‑protein transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/26); total (20/26) |
ACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCA > NC_000913/927083‑927328
|
aCCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTg > 1:158514/1‑139 (MQ=255)
acgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt < 2:861141/139‑1 (MQ=255)
acgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt < 2:180960/139‑1 (MQ=255)
ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGa > 1:1023595/1‑139 (MQ=255)
ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGa > 1:43539/1‑139 (MQ=255)
cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg > 2:1004243/1‑139 (MQ=255)
cgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCg < 2:106793/139‑1 (MQ=255)
aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac < 2:518461/139‑1 (MQ=255)
aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac > 2:896677/1‑139 (MQ=255)
cGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGacca > 2:556393/1‑139 (MQ=255)
tGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca < 2:158514/139‑1 (MQ=255)
tGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGCGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc < 1:662995/131‑1 (MQ=255)
tGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCATGACACGATCGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc > 2:662995/1‑131 (MQ=255)
acaGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAgg < 1:1004243/139‑1 (MQ=255)
caGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCAGGGATCGGGCGACCACCAGAGGa < 2:43539/139‑1 (MQ=255)
ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg < 2:308705/122‑1 (MQ=255)
ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg > 1:308705/1‑122 (MQ=255)
ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgg > 2:561259/1‑139 (MQ=255)
cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTGTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTgg > 2:1045620/1‑139 (MQ=255)
ctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGga < 1:556393/139‑1 (MQ=255)
gccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa > 2:669775/2‑139 (MQ=255)
accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGGTATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca > 1:669704/1‑117 (MQ=255)
accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGaccacct < 2:669704/117‑2 (MQ=255)
tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt > 2:238684/1‑72 (MQ=255)
tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt < 1:238684/72‑1 (MQ=255)
aTCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATAc < 2:1023595/139‑1 (MQ=255)
cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt < 2:681428/64‑1 (MQ=255)
cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt > 1:681428/1‑64 (MQ=255)
cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa < 1:466130/74‑1 (MQ=255)
cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa > 2:466130/1‑74 (MQ=255)
cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCa < 2:969358/139‑1 (MQ=255)
cGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCTCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt < 1:669775/139‑1 (MQ=255)
cGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt < 1:896677/139‑1 (MQ=255)
gATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt < 2:216841/54‑1 (MQ=255)
gATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt > 1:216841/1‑54 (MQ=255)
aTAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGa > 2:1002284/1‑84 (MQ=255)
aTAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGa < 1:1002284/84‑1 (MQ=255)
tAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAg > 2:1057261/1‑139 (MQ=255)
tAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAg < 1:63093/139‑1 (MQ=255)
ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg > 1:1028774/1‑44 (MQ=255)
ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg < 2:1028774/44‑1 (MQ=255)
gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac > 1:752748/1‑76 (MQ=255)
gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac < 2:752748/76‑1 (MQ=255)
gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc < 1:1057261/139‑1 (MQ=255)
gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc < 1:1045620/139‑1 (MQ=255)
aaaTCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCa < 1:940765/139‑1 (MQ=255)
|
ACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCA > NC_000913/927083‑927328
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
