Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	927,205	A→G	R76R (CGT→CGC)	aat ←	leucyl/phenylalanyl‑tRNA‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	927,205	0	A	G	100.0%	161.5 / NA	47	R76R (CGT→CGC)	aat	leucyl/phenylalanyl‑tRNA‑protein transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/23); total (24/23)

ATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGA  >  NC_000913/927070‑927337

aTGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                                   <  2:292751/139‑1 (MQ=255)

aTGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                                   <  1:450511/139‑1 (MQ=255)

         ttCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGc                                                                                                                          <  2:986801/139‑1 (MQ=255)

           cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGcgg                                                                                                                        >  1:1212983/1‑137 (MQ=255)

           cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                                        >  2:310286/1‑139 (MQ=255)

                      cgcgTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGcc                                                                                                             <  2:1238822/139‑1 (MQ=255)

                            aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                                   <  1:393426/127‑1 (MQ=255)

                            aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                                   >  2:393426/1‑127 (MQ=255)

                             tCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                      <  2:739454/139‑1 (MQ=255)

                                 ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                                   <  2:91609/106‑1 (MQ=255)

                                 ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                                   >  1:91609/1‑106 (MQ=255)

                                 ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcg                                                                                                  <  1:310286/139‑1 (MQ=255)

                                 ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcg                                                                                                  >  1:547764/1‑139 (MQ=255)

                                       ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcgcg                                                                                                >  2:802850/1‑135 (MQ=255)

                                       ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcgcg                                                                                                <  1:802850/135‑1 (MQ=255)

                                       ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGa                                                                                            >  2:50954/1‑139 (MQ=255)

                                        tcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGAt                                                                                           >  1:329683/1‑139 (MQ=255)

                                            cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGata                                                                                                                             >  1:809892/1‑101 (MQ=255)

                                            cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGata                                                                                                                             <  2:809892/101‑1 (MQ=255)

                                                aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                                   <  2:547764/139‑1 (MQ=255)

                                                aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                                   >  2:1151517/1‑139 (MQ=255)

                                                     tGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCGGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca                                                                              <  2:1212983/139‑1 (MQ=255)

                                                                       cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGTGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTgg                                                            >  1:1455814/1‑139 (MQ=255)

                                                                       cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTgg                                                            >  1:1298417/1‑139 (MQ=255)

                                                                           accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa                                                        >  2:485093/1‑139 (MQ=255)

                                                                           accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa                                                        >  2:1124207/1‑139 (MQ=255)

                                                                           accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa                                                        >  2:887291/1‑139 (MQ=255)

                                                                           accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa                                                        <  2:1298417/139‑1 (MQ=255)

                                                                                aaGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCa                                                   <  1:1151517/139‑1 (MQ=255)

                                                                                  gCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACg                                                 <  2:329683/139‑1 (MQ=255)

                                                                                             cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCa                                      >  1:1462850/1‑139 (MQ=255)

                                                                                              gTGACACGATAGGGCGAGTGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCAc                                     <  2:1455814/139‑1 (MQ=255)

                                                                                                 acacGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGata                                                                                                                             >  2:724634/1‑48 (MQ=255)

                                                                                                 acacGATAGGGCGAGCGTTTATGAAATCGCTTCAGACTGCGGCTGata                                                                                                                             <  1:724634/48‑1 (MQ=38)

                                                                                                  cacGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTg                                 >  1:766240/1‑139 (MQ=255)

                                                                                                       tAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAg                            <  1:485093/139‑1 (MQ=255)

                                                                                                         gggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                               >  1:1471169/1‑134 (MQ=255)

                                                                                                         gggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                               <  2:1471169/134‑1 (MQ=255)

                                                                                                            cGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCa                                      <  2:337777/124‑1 (MQ=255)

                                                                                                            cGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCa                                      >  1:337777/1‑124 (MQ=255)

                                                                                                              aGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTaa                     >  1:595526/1‑139 (MQ=255)

                                                                                                               gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca                                                                              <  2:437860/81‑1 (MQ=255)

                                                                                                               gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca                                                                              >  1:437860/1‑81 (MQ=255)

                                                                                                                           tCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAggg        >  2:1137610/1‑139 (MQ=255)

                                                                                                                            cGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGc       >  2:93525/1‑139 (MQ=255)

                                                                                                                                 cATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGa  <  1:93525/139‑1 (MQ=255)

                                                                                                                                 cATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGa  <  2:766240/139‑1 (MQ=255)

ATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGA  >  NC_000913/927070‑927337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A