Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,082,896	T→A	R218R (CGT→CGA)	efeO →	inactive ferrous ion transporter EfeUOB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,082,896	0	T	A	100.0%	125.6 / NA	40	R218R (CGT→CGA)	efeO	inactive ferrous ion transporter EfeUOB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (18/22); total (18/22)

CGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAG  >  NC_000913/1082769‑1083020

cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGc                                                                                                                   >  1:236013/1‑139 (MQ=255)

cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGc                                                                                                                   >  1:756383/1‑139 (MQ=255)

    ccAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTg                                                                                                               >  2:370779/1‑139 (MQ=255)

     cAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGt                                                                                                              <  2:236013/139‑1 (MQ=255)

               aGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGacaa                                                                                                    <  2:236061/139‑1 (MQ=255)

                     ttGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcaccac                                                                                              >  2:1049570/1‑139 (MQ=255)

                       gAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGC‑AAAAGCCACCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCaa                                                                                           <  1:267742/139‑1 (MQ=255)

                        aaCCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAGAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCaa                                                                                           <  2:350479/139‑1 (MQ=255)

                        aaCCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCaa                                                                                           <  1:132981/139‑1 (MQ=255)

                        aaCCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCaa                                                                                           <  1:475774/139‑1 (MQ=255)

                          ccGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAg                                                                                         <  2:382805/139‑1 (MQ=255)

                                         ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcacc                                                                                                <  2:740440/117‑1 (MQ=255)

                                         ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcacc                                                                                                >  1:740440/1‑117 (MQ=255)

                                         ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACg                                                                          >  1:400993/1‑139 (MQ=255)

                                         ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACg                                                                          >  2:918602/1‑139 (MQ=255)

                                              cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCa                                                                               >  1:200406/1‑129 (MQ=255)

                                              cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCa                                                                               <  2:200406/129‑1 (MQ=255)

                                              cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGag                                                                     <  1:918602/139‑1 (MQ=255)

                                              cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGag                                                                     <  1:1049570/139‑1 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCAt                                                                                                                 <  1:774523/90‑1 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCAt                                                                                                                 >  2:774523/1‑90 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGac                                                                                                      <  1:254539/101‑1 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGac                                                                                                      >  2:254539/1‑101 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACAccacca                                                                                            >  1:1024375/1‑111 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACAccacca                                                                                            <  2:1024375/111‑1 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCt                                                                <  1:419149/139‑1 (MQ=255)

                                                   tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCt                                                                >  2:280148/1‑139 (MQ=255)

                                                                         tGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTg                                          >  1:52960/1‑139 (MQ=255)

                                                                                  ttACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCa                                                                                                                  <  2:966259/58‑1 (MQ=255)

                                                                                  ttACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCa                                                                                                                  >  1:966259/1‑58 (MQ=255)

                                                                                   tACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCa                                >  2:418510/1‑139 (MQ=255)

                                                                                     cGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACAccacca                                                                                            <  1:827451/77‑1 (MQ=255)

                                                                                     cGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACAccacca                                                                                            >  2:827451/1‑77 (MQ=255)

                                                                                      gAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCa                             <  1:370779/139‑1 (MQ=255)

                                                                                           aaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACg                                  >  1:845700/1‑129 (MQ=255)

                                                                                           aaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACg                                  <  2:845700/129‑1 (MQ=255)

                                                                                                      aCCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTccc             <  2:400993/139‑1 (MQ=255)

                                                                                                      aCCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTccc             <  2:756383/139‑1 (MQ=255)

                                                                                                       cccAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCa            <  1:855147/139‑1 (MQ=255)

                                                                                                                 aCTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAg  >  1:267224/1‑139 (MQ=255)

CGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAG  >  NC_000913/1082769‑1083020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A