Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,082,896	T→A	R218R (CGT→CGA)	efeO →	inactive ferrous ion transporter EfeUOB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,082,896	0	T	A	100.0%	95.2 / NA	31	R218R (CGT→CGA)	efeO	inactive ferrous ion transporter EfeUOB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (15/16); total (15/16)

ACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAA  >  NC_000913/1082764‑1083017

aCCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGaa                                                                                                                     >  2:438303/1‑139 (MQ=255)

     cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGc                                                                                                                >  1:178358/1‑139 (MQ=255)

     cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGc                                                                                                                >  1:614512/1‑139 (MQ=255)

      gcgcCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCa                                                                                                               <  1:718074/139‑1 (MQ=255)

                tATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCg                                                                                                     <  2:614512/139‑1 (MQ=255)

                      cGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGacaaca                                                                                               <  1:313822/139‑1 (MQ=255)

                              aCCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGGCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGac                                                                                                   >  1:566437/1‑127 (MQ=255)

                              aCCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGac                                                                                                   <  2:566437/127‑1 (MQ=255)

                                    tGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg                                                                                 <  2:537022/139‑1 (MQ=255)

                                    tGCTGAACTGTTCTCAGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg                                                                                 >  1:537022/1‑139 (MQ=255)

                                              ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCTGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCaa                                                                                        >  1:137320/1‑122 (MQ=255)

                                              ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCaa                                                                                        <  2:137320/122‑1 (MQ=255)

                                                    gATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                 <  1:253151/139‑1 (MQ=255)

                                                     aTCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAggg                                                                                    <  1:205884/119‑1 (MQ=255)

                                                     aTCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAggg                                                                                    >  2:205884/1‑119 (MQ=255)

                                                        tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                 >  1:753081/1‑135 (MQ=255)

                                                        tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                 <  2:753081/135‑1 (MQ=255)

                                                        tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCt                                                             >  1:228455/1‑139 (MQ=255)

                                                        tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCt                                                             <  1:438303/139‑1 (MQ=255)

                                                               cagcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATAcc                                                      <  2:157496/139‑1 (MQ=255)

                                                               cagcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATAcc                                                      >  1:157496/1‑139 (MQ=255)

                                                               cagcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATAcc                                                      >  2:258790/1‑139 (MQ=255)

                                                                    ttGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGAtgt                                                 >  1:352855/1‑139 (MQ=255)

                                                                            cGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGAtt                                         >  2:685269/1‑139 (MQ=255)

                                                                             gTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGAttt                                        <  2:228455/139‑1 (MQ=255)

                                                                                 aGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaa                                    <  2:352855/139‑1 (MQ=255)

                                                                                          cGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATc                           >  2:94106/1‑139 (MQ=255)

                                                                                           gAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCa                          <  1:94106/139‑1 (MQ=255)

                                                                                             gCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGt                        >  2:188268/1‑139 (MQ=255)

                                                                                                            cccAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCa         <  2:178358/139‑1 (MQ=255)

                                                                                                                   ttCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCaa  <  1:685269/139‑1 (MQ=255)

ACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAA  >  NC_000913/1082764‑1083017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A