Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,260,528 A→G I92I (ATT→ATC)  dauA ← C4‑dicarboxylic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,260,5280AG100.0% 98.3 / NA 28I92I (ATT→ATC) dauAC4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

ACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCA  >  NC_000913/1260416‑1260655
                                                                                                                |                                                                                                                               
aCTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGTGATAGAGGATTACCACAAATGCCGCAGt                                                                                                             <  1:664684/133‑1 (MQ=255)
aCTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                             >  2:664684/1‑133 (MQ=255)
    aaTCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa                                                                                                   >  1:530212/1‑139 (MQ=255)
               cAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg                                                                                        <  2:446843/139‑1 (MQ=255)
                        ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGacccacc                                                                               >  1:20731/1‑139 (MQ=255)
                                aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       >  2:331354/1‑139 (MQ=255)
                                aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  1:1033256/139‑1 (MQ=255)
                                aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  2:1040667/139‑1 (MQ=255)
                                       tCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGAc                                                                >  2:781394/1‑139 (MQ=255)
                                                 ccccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCa                                                      >  1:226852/1‑139 (MQ=255)
                                                 ccccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCa                                                      >  2:225891/1‑139 (MQ=255)
                                                     gACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAg                                                  <  2:20731/139‑1 (MQ=255)
                                                         gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                                              <  2:136624/139‑1 (MQ=255)
                                                          cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                             <  1:61820/139‑1 (MQ=255)
                                                          cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                             <  1:331354/139‑1 (MQ=255)
                                                                  cAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATc                                                            >  1:643650/1‑116 (MQ=255)
                                                                  cAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATc                                                            <  2:643650/116‑1 (MQ=255)
                                                                   aaCCAGCAGTCCTGCCAGTCCAAACTGTTGTGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaaa                                    <  1:577851/139‑1 (MQ=255)
                                                                           gTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                            <  1:781394/139‑1 (MQ=255)
                                                                            tCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgg                           <  2:660144/139‑1 (MQ=255)
                                                                            tCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgg                           >  2:70560/1‑139 (MQ=255)
                                                                              cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgggg                         <  2:530212/139‑1 (MQ=255)
                                                                              cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgggg                         <  2:821497/139‑1 (MQ=255)
                                                                                gCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAGGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTg                       >  1:713088/1‑139 (MQ=255)
                                                                                 ccAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGc                      <  2:685437/139‑1 (MQ=255)
                                                                                     tCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca                  >  2:907941/1‑139 (MQ=255)
                                                                                               tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa        >  2:71698/1‑139 (MQ=255)
                                                                                                     acGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCa  <  1:70560/139‑1 (MQ=255)
                                                                                                                |                                                                                                                               
ACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCA  >  NC_000913/1260416‑1260655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A