Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,528	A→G	I92I (ATT→ATC)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,528	0	A	G	97.2%	122.5 / ‑6.2	36	I92I (ATT→ATC)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/16); minor base C (0/1); total (19/17)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAA  >  NC_000913/1260410‑1260649

gAATATACTCAATCAGGCGACCAAAGCGTGCCAGACGCATCAGAATCAAAAAGATCCCCGACTGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       <  2:964193/139‑1 (MQ=255)

        tCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCgg                                                                                               >  2:970861/1‑139 (MQ=255)

               ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa                                                                                             <  2:723710/134‑1 (MQ=255)

               ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa                                                                                             >  1:723710/1‑134 (MQ=255)

               ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                        >  2:601125/1‑139 (MQ=255)

               ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                        >  1:1099174/1‑139 (MQ=255)

                gCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa                                                                                       <  1:709432/139‑1 (MQ=255)

                   acgaaaGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCCGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAg                                                                                    <  2:966420/136‑1 (MQ=255)

                    ccAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCg                                                                                                           <  2:693571/115‑1 (MQ=255)

                    ccAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCg                                                                                                           >  1:693571/1‑115 (MQ=255)

                    ccAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGc                                                                                   >  1:897072/1‑139 (MQ=255)

                                   cccattaggaataaaaaaATCCCCGGAAGCAAGGGCGCAACCAGCAGTCCTGCCCGTTCCAAACTTTGCCGAAACGGGTAGGGGATTTCCCCCAATTGCCG‑AGGCGGACCGGGAACGCCAAAACGGGACCCAACCGACa                                                                    >  2:1287178/13‑139 (MQ=255)

                                        cAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCa                                                               >  1:969113/1‑139 (MQ=255)

                                              cAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                                         <  2:969113/139‑1 (MQ=255)

                                                     aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                                      >  1:1260551/1‑119 (MQ=255)

                                                     aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                                      >  2:665233/1‑119 (MQ=255)

                                                     aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                                      <  1:665233/119‑1 (MQ=255)

                                                     aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                                      <  2:1260551/119‑1 (MQ=255)

                                                     aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCg                                                  >  1:663593/1‑139 (MQ=255)

                                                               gCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                 >  1:582153/1‑114 (MQ=255)

                                                               gCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                 <  2:582153/114‑1 (MQ=255)

                                                                       gCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGac                                                                              >  2:139114/1‑93 (MQ=255)

                                                                       gCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGac                                                                              <  1:139114/93‑1 (MQ=255)

                                                                        cAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                           >  2:89587/1‑95 (MQ=255)

                                                                        cAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                           <  1:89587/95‑1 (MQ=255)

                                                                               cagTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGa                                                                                              >  1:782130/1‑69 (MQ=255)

                                                                               cagTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGCATTACCACAAATGCCGCAGTCGGACCAGa                                                                                              <  2:782130/69‑1 (MQ=255)

                                                                                       ccAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCaa                                               >  2:482502/1‑108 (MQ=255)

                                                                                       ccAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCaa                                               <  1:482502/108‑1 (MQ=255)

                                                                                         aG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCca              <  1:1145549/139‑1 (MQ=255)

                                                                                             ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                      <  2:1217242/128‑1 (MQ=255)

                                                                                             ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                      >  1:1217242/1‑128 (MQ=255)

                                                                                                aaCTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCAcaccac        <  2:663593/139‑1 (MQ=255)

                                                                                                aaCTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCAcaccac        >  1:1162164/1‑139 (MQ=255)

                                                                                                     ttGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg                                                                                  >  2:675816/1‑60 (MQ=255)

                                                                                                     ttGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg                                                                                  <  1:675816/60‑1 (MQ=255)

                                                                                                      tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa  >  1:777693/1‑139 (MQ=255)

GAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAA  >  NC_000913/1260410‑1260649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A