Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,420,793 |
(G)6→7 |
coding (110/423 nt) |
ydaT → |
Rac prophage, uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,420,787 | 1 | . | G | 100.0%
| 151.8
/ NA
| 43 | Q35R (CAG→CGG) | ydaT | Rac prophage, uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (22/21); total (22/21) |
TCATACCTGAAACATCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑GGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTCAGAAGCTGATCCCAGCAATTCT > NC_000913/1420652‑1420916
|
tCATACCTGAAACATCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑ggg > 1:495268/1‑139 (MQ=255)
aaCATCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGa > 2:581259/1‑139 (MQ=255)
aaCATCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑gggggg < 2:791442/132‑1 (MQ=255)
aaCATCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑gggggg > 1:791442/1‑132 (MQ=255)
tCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑gggggg > 1:276292/1‑128 (MQ=255)
tCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑gggggg < 2:276292/128‑1 (MQ=255)
agGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCt < 2:907158/139‑1 (MQ=255)
agGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCt < 1:224201/139‑1 (MQ=255)
cAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGt < 2:495268/139‑1 (MQ=255)
tCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCa < 1:106021/139‑1 (MQ=255)
tCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCa < 1:1068297/139‑1 (MQ=255)
aTGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAg < 1:581259/139‑1 (MQ=255)
aaTCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTg < 2:134585/139‑1 (MQ=255)
aGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcagc > 2:49144/1‑139 (MQ=255)
acaTCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGa < 1:49144/139‑1 (MQ=255)
aTCGAATCAGTGTTGTTTGCCCTAACAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAat > 2:336743/1‑139 (MQ=255)
tgttgtTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc > 2:153348/1‑139 (MQ=255)
tgttgtTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc > 1:989757/1‑139 (MQ=255)
ttgttTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgt > 1:788408/1‑139 (MQ=255)
ccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGtt > 1:705810/1‑89 (MQ=255)
ccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGtt < 2:705810/89‑1 (MQ=255)
ccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGc < 1:662026/95‑1 (MQ=255)
ccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGc > 2:662026/1‑95 (MQ=255)
agcagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAAt > 2:1000283/1‑107 (MQ=255)
agcagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAAt < 1:1000283/107‑1 (MQ=255)
agcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGa < 2:788408/139‑1 (MQ=255)
ggTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag < 1:977683/86‑1 (MQ=255)
ggTAGCCAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag > 2:977683/1‑86 (MQ=255)
ccAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag < 2:390436/81‑1 (MQ=255)
ccAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag > 1:390436/1‑81 (MQ=255)
ccAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTc > 2:940054/1‑139 (MQ=255)
ccAATGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTc > 1:363498/1‑139 (MQ=255)
ccAATGCAATTACTG‑AGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc < 1:692112/96‑1 (MQ=255)
ccAATGCAATTACTG‑AGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc > 2:692112/1‑96 (MQ=255)
tGCAATTACTGAAGTATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGaa < 2:627541/82‑1 (MQ=255)
tGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGa > 2:320919/1‑69 (MQ=255)
tGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGa < 1:320919/69‑1 (MQ=255)
tGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcagca > 2:677915/1‑79 (MQ=255)
tGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcagca < 1:677915/79‑1 (MQ=255)
tGCAATTACTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGaa > 1:627541/1‑82 (MQ=255)
aCTGAAGAATATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTCAGAAGCTGATc > 1:948499/1‑139 (MQ=255)
gaagaaTATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTCAGAAGCTGATCCCa > 2:70704/1‑139 (MQ=255)
gaagaaTATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTCAGAAGCTGATCCCa < 2:948499/139‑1 (MQ=255)
gaaTATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAg > 2:177652/1‑49 (MQ=39)
gaaTATCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAg < 1:177652/49‑1 (MQ=39)
tCTGCGCCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTCAGAAGCTGATCCCAGCAATTCt > 1:814301/1‑139 (MQ=255)
gcgcCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgt > 1:444577/1‑74 (MQ=255)
gcgcCAGGGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgt < 2:444577/74‑1 (MQ=255)
|
TCATACCTGAAACATCAAGAGGCAAATGATTCATGAAAATCAAGCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑GGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGAAAAAATTCAGAAGCTGATCCCAGCAATTCT > NC_000913/1420652‑1420916
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
