Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,581	G→A	G295R (GGA→AGA)	ynfM →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,581	0	G	A	100.0%	105.7 / NA	34	G295R (GGA→AGA)	ynfM	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/15); total (19/15)

GGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGA  >  NC_000913/1670453‑1670715

ggTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGac                                                                                                                              <  1:604999/139‑1 (MQ=255)

                    aaTTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                          <  2:1054237/139‑1 (MQ=255)

                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCa                                                                                                        >  2:328434/1‑134 (MQ=255)

                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCa                                                                                                        <  1:328434/134‑1 (MQ=255)

                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                                   >  2:537687/1‑139 (MQ=255)

                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                                   >  1:35389/1‑139 (MQ=255)

                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                                   >  2:178063/1‑139 (MQ=255)

                              gCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtgtt                                                                                                >  1:681089/1‑139 (MQ=255)

                                  tCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAGAGCCAGAACCATGacca                                                                                                                            <  2:852655/107‑1 (MQ=255)

                                  tCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAGAGCCAGAACCATGacca                                                                                                                            >  1:852655/1‑107 (MQ=255)

                                    ggTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:750320/118‑1 (MQ=255)

                                    ggTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:750320/1‑118 (MQ=255)

                                       tGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                       <  1:825140/139‑1 (MQ=255)

                                                 ccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTTACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                             <  1:949220/139‑1 (MQ=255)

                                                 ccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                             <  1:178063/139‑1 (MQ=255)

                                                           gtcagtcaGGCGGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGGTCACACAACGCCAGAACGGTGACGCCCCCCCATGGGCGGGGTCGAGTGATGGTGGTTTCGTCGGGGGTTATGCTggtggctt                                                                   >  2:639037/1‑132 (MQ=255)

                                                           gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                        >  1:547729/1‑134 (MQ=255)

                                                           gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                        <  2:547729/134‑1 (MQ=255)

                                                           gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGtt                                                                   >  1:232721/1‑139 (MQ=255)

                                                            tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                       <  1:360848/118‑1 (MQ=255)

                                                            tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                       >  2:360848/1‑118 (MQ=255)

                                                                 cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                             <  2:35389/139‑1 (MQ=255)

                                                                              gCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:431195/1‑76 (MQ=255)

                                                                              gCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:431195/76‑1 (MQ=255)

                                                                                    tatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCt                                          >  2:1046055/1‑139 (MQ=255)

                                                                                     atCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCtg                                         >  1:234786/1‑139 (MQ=255)

                                                                                                 tttGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCttt                             >  1:114487/1‑139 (MQ=255)

                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:606290/39‑1 (MQ=255)

                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:606290/1‑39 (MQ=255)

                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCATATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttc           >  2:218465/1‑139 (MQ=255)

                                                                                                                    tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct          >  2:1049964/1‑139 (MQ=255)

                                                                                                                      aCCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               <  2:601980/36‑1 (MQ=255)

                                                                                                                      aCCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                               >  1:601980/1‑36 (MQ=255)

                                                                                                                            aGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGa  <  1:1046055/139‑1 (MQ=255)

GGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGA  >  NC_000913/1670453‑1670715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A