Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,419,456	T→C	N57S (AAC→AGC)	ulaG ←	L‑ascorbate 6‑phosphate lactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,419,456	0	T	C	100.0%	152.2 / NA	43	N57S (AAC→AGC)	ulaG	L‑ascorbate 6‑phosphate lactonase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/22); total (21/22)

ACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCA  >  NC_000913/4419324‑4419585

aCAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGc                                                                                                                             <  1:515154/139‑1 (MQ=255)

   aaCGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCAc                                                                                                                          >  2:398070/1‑139 (MQ=255)

       gggTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTc                                                                                                                      <  2:429476/139‑1 (MQ=255)

         gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCg                                                                                                                    >  2:900469/1‑139 (MQ=255)

             tACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGAttt                                                                                                                <  1:900469/139‑1 (MQ=255)

                  aGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAAcc                                                                                                           >  1:510178/1‑139 (MQ=255)

                      ttGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGAt                                                                                                       >  1:23206/1‑139 (MQ=255)

                        ggCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATcc                                                                                                     <  2:510178/139‑1 (MQ=255)

                                ttttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCag                                                                                             >  2:475878/1‑139 (MQ=255)

                                 tttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            <  2:528843/139‑1 (MQ=255)

                                 tttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            <  1:475878/139‑1 (MQ=255)

                                                    gcTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                         <  1:765771/139‑1 (MQ=255)

                                                        cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAAtgtg                                                                     >  2:495892/1‑139 (MQ=255)

                                                        cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAAtgtg                                                                     >  1:771272/1‑139 (MQ=255)

                                                           cTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagccaagc                                                                                       <  2:337641/118‑1 (MQ=255)

                                                           cTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagccaagc                                                                                       >  1:337641/1‑118 (MQ=255)

                                                           cTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCa                                                                  <  2:990782/139‑1 (MQ=255)

                                                              gTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGa                                                               <  1:398070/139‑1 (MQ=255)

                                                                   ccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                          >  1:503809/1‑139 (MQ=255)

                                                                        ttCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGtt                                                     >  1:648345/1‑139 (MQ=255)

                                                                            ttAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTg                                                 >  1:781585/1‑139 (MQ=255)

                                                                                      aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                          >  1:181681/1‑120 (MQ=255)

                                                                                      aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                          <  2:181681/120‑1 (MQ=255)

                                                                                        cGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                         <  1:755351/103‑1 (MQ=255)

                                                                                        cGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                         >  2:755351/1‑103 (MQ=255)

                                                                                        cGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATttcttc                                     <  1:968460/139‑1 (MQ=255)

                                                                                             cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGAt                                                                                                                  <  2:837479/57‑1 (MQ=255)

                                                                                             cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGAt                                                                                                                  >  1:837479/1‑57 (MQ=255)

                                                                                             cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCttcattca                                <  1:526474/139‑1 (MQ=255)

                                                                                              tttgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCaa                               <  2:227115/139‑1 (MQ=255)

                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCa                                                                                                          >  1:746068/1‑52 (MQ=255)

                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCa                                                                                                          <  2:746068/52‑1 (MQ=255)

                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            >  1:198312/1‑66 (MQ=255)

                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            <  2:198312/66‑1 (MQ=255)

                                                                                                            ccGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCaa                                                                                                             <  1:744216/47‑1 (MQ=255)

                                                                                                            ccGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCaa                                                                                                             >  2:744216/1‑47 (MQ=255)

                                                                                                            ccGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACt                 >  1:1060680/1‑139 (MQ=255)

                                                                                                               cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCg              >  2:982920/1‑139 (MQ=255)

                                                                                                               cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCg              >  2:132377/1‑139 (MQ=255)

                                                                                                                    gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGaaa         >  2:704740/1‑139 (MQ=255)

                                                                                                                    gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGaaa         <  2:648345/139‑1 (MQ=255)

                                                                                                                           aCGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCa  <  1:982920/139‑1 (MQ=255)

                                                                                                                           aCGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCa  <  2:1060680/139‑1 (MQ=255)

ACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCA  >  NC_000913/4419324‑4419585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A