Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,419,456 |
T→C |
N57S (AAC→AGC) |
ulaG ← |
L‑ascorbate 6‑phosphate lactonase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,419,456 | 0 | T | C | 100.0%
| 182.8
/ NA
| 51 | N57S (AAC→AGC) | ulaG | L‑ascorbate 6‑phosphate lactonase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/25); total (26/25) |
AACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTC > NC_000913/4419327‑4419584
|
aaCGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCAc > 1:1110962/1‑139 (MQ=255)
gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCg > 2:1044564/1‑139 (MQ=255)
aCGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTc < 1:1044564/139‑1 (MQ=255)
aGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGc < 2:1138688/139‑1 (MQ=255)
tttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc < 2:443331/139‑1 (MQ=255)
tttCACGCCAGCCATGCGCTGCATCTGGTGTCCCTGTTTGATTAACGGGTTACCGTGACGTTGTTTGCCAATGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagcct > 2:738773/1‑138 (MQ=255)
ccagccaTGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGccacc > 2:545923/1‑139 (MQ=255)
gcTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa < 2:176589/139‑1 (MQ=255)
cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagcca < 2:176564/118‑1 (MQ=255)
cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagcca > 1:176564/1‑118 (MQ=255)
cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc > 1:613260/1‑116 (MQ=255)
cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc < 2:613260/116‑1 (MQ=255)
tCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGcc > 2:731425/1‑139 (MQ=255)
cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTc < 1:411957/80‑1 (MQ=255)
cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTc > 2:411957/1‑80 (MQ=255)
cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTc < 1:905912/87‑1 (MQ=255)
cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTc > 2:905912/1‑87 (MQ=255)
cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGa > 2:775950/1‑139 (MQ=255)
ccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc > 2:349488/1‑139 (MQ=255)
tttCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAg > 2:698104/1‑131 (MQ=255)
tttCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAg < 1:698104/131‑1 (MQ=255)
tAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGt > 1:1177530/1‑139 (MQ=255)
aCGGTTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTc < 1:967689/139‑1 (MQ=255)
gggTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGcc < 2:586556/59‑1 (MQ=255)
gggTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGcc > 1:586556/1‑59 (MQ=255)
gTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCa > 2:1006488/1‑106 (MQ=255)
gTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCa < 1:1006488/106‑1 (MQ=255)
aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTc < 1:374105/126‑1 (MQ=255)
aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTc > 2:374105/1‑126 (MQ=255)
cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCttcattca < 2:1177530/139‑1 (MQ=255)
tttgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCaa < 2:77978/139‑1 (MQ=255)
tttgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCaa < 1:731425/139‑1 (MQ=255)
tgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTTCGATTTCAACCAGATCCCGGTTCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTGCAATTTCTTCATTCAAcc < 1:738773/139‑1 (MQ=255)
tgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTTTTCAATTTCTTCATTCAACCAGCt < 2:910903/139‑1 (MQ=255)
gCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGTAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTa > 2:619623/1‑139 (MQ=255)
ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGc > 1:692663/1‑94 (MQ=255)
ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGc < 2:692663/94‑1 (MQ=255)
ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAg < 2:1315005/135‑1 (MQ=255)
ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAg > 1:1315005/1‑135 (MQ=255)
cACCAGAAATCAACGCAAACGCTGGTGCCACCTTc < 1:798635/35‑1 (MQ=255)
cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc > 2:295161/1‑61 (MQ=255)
cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc < 1:295161/61‑1 (MQ=255)
cACCAGAAATCAACGCAAACGCTGGTGCCACCATc > 2:798635/1‑35 (MQ=25)
gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCg < 2:1292603/88‑1 (MQ=255)
gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCg > 1:1292603/1‑88 (MQ=255)
gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGaaa > 2:1324520/1‑139 (MQ=255)
aaCGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTc > 2:1020585/1‑139 (MQ=255)
cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc > 1:997997/1‑48 (MQ=255)
cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc < 2:997997/48‑1 (MQ=255)
cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa > 1:338670/1‑67 (MQ=255)
cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa < 2:338670/67‑1 (MQ=255)
|
AACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTC > NC_000913/4419327‑4419584
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
