Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,464,629 |
G→A |
S27S (AGC→AGT) |
treC ← |
trehalose‑6‑P hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,464,629 | 0 | G | A | 100.0%
| 114.6
/ NA
| 36 | S27S (AGC→AGT) | treC | trehalose‑6‑P hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/17); total (19/17) |
CGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGC > NC_000913/4464506‑4464745
|
cGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTcc > 2:865487/1‑139 (MQ=255)
tACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGa > 1:783933/1‑139 (MQ=255)
tACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGa > 1:660103/1‑139 (MQ=255)
aaCCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCtt < 1:814020/139‑1 (MQ=255)
ttATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt < 1:305155/139‑1 (MQ=255)
cTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa > 2:518127/1‑130 (MQ=255)
cTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa < 1:518127/130‑1 (MQ=255)
tAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGt > 1:383610/1‑139 (MQ=255)
gTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc < 2:816293/139‑1 (MQ=255)
aGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTgggg < 2:383610/139‑1 (MQ=255)
aGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTgggg < 1:865487/139‑1 (MQ=255)
aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGa > 1:35748/1‑139 (MQ=255)
aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccac < 2:390261/103‑1 (MQ=255)
aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccac > 1:390261/1‑103 (MQ=255)
tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa > 1:818292/1‑75 (MQ=255)
tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa < 2:818292/75‑1 (MQ=255)
tCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAAc < 2:440346/86‑1 (MQ=255)
tCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAAc > 1:440346/1‑86 (MQ=255)
tgtTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCt > 1:425732/1‑125 (MQ=255)
tgtTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCt < 2:425732/125‑1 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCgtg < 1:309/38‑1 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCgtg > 2:309/1‑38 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc > 1:376743/1‑90 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc < 2:376743/90‑1 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt < 2:694490/109‑1 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt > 1:694490/1‑109 (MQ=255)
ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGgcgc > 1:856059/1‑139 (MQ=255)
aaCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt > 2:865041/1‑105 (MQ=255)
aaCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt < 1:865041/105‑1 (MQ=255)
aCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGaaa > 2:85308/1‑44 (MQ=255)
aCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGaaa < 1:85308/44‑1 (MQ=255)
cGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa < 1:126739/53‑1 (MQ=255)
cGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa > 2:126739/1‑53 (MQ=255)
cGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTgc > 2:602764/1‑139 (MQ=255)
tCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGAt < 2:314540/85‑1 (MQ=255)
tCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGAt > 1:314540/1‑85 (MQ=255)
|
CGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGC > NC_000913/4464506‑4464745
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
