Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,595,527 |
T→C |
T109A (ACG→GCG) |
lgoR ← |
putative transcriptional activator for L‑galactonate catabolism |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,595,527 | 0 | T | C | 94.4%
| 108.4
/ ‑2.7
| 36 | T109A (ACG→GCG) | lgoR | putative transcriptional activator for L‑galactonate catabolism |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (18/16); total (19/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 |
CGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACG > NC_000913/4595395‑4595647
|
cGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTcccc < 1:62814/139‑1 (MQ=255)
cATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAAc < 2:897022/139‑1 (MQ=255)
aTAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTcccc > 1:834485/1‑98 (MQ=255)
aTAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTcccc < 2:834485/98‑1 (MQ=255)
aTTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCa > 2:76380/1‑139 (MQ=255)
tataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaaa > 2:397550/1‑139 (MQ=255)
taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAATGCCTGCTCcaa < 2:622896/137‑1 (MQ=255)
taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaaa > 1:622896/1‑137 (MQ=255)
taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTccc > 1:582900/1‑88 (MQ=255)
taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTccc < 2:582900/88‑1 (MQ=255)
taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca > 2:671670/1‑139 (MQ=255)
taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca > 1:144577/1‑139 (MQ=255)
cGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACt > 1:1006141/1‑97 (MQ=255)
cGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACt < 2:1006141/97‑1 (MQ=255)
agcagatagctcgTACTACAACTGGCCCCACACCCGCGGCCCAAGCCAGCTGCAGTTCAGAAAACTCTTTCCCGCGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTATGTTTTGTTCACt < 2:267261/130‑1 (MQ=255)
atTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa < 1:364809/139‑1 (MQ=255)
actacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtag > 2:869675/1‑139 (MQ=255)
actacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtag > 1:106335/1‑139 (MQ=255)
aCCGGGCTGACACCCGCGGCCCGCGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGATTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTAAAGCgactgtcactt > 1:267261/1‑129 (MQ=255)
cGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCg < 1:397550/139‑1 (MQ=255)
cGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCg > 2:202901/1‑139 (MQ=255)
gggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGa < 1:76380/139‑1 (MQ=255)
ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTcccc < 2:962148/49‑1 (MQ=255)
ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTcccc > 1:962148/1‑49 (MQ=255)
ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa > 2:372262/1‑121 (MQ=255)
ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa < 1:372262/121‑1 (MQ=255)
gCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc > 2:300679/1‑88 (MQ=255)
gCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc < 1:300679/88‑1 (MQ=255)
acacCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGt > 2:491842/1‑139 (MQ=255)
cccGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc < 2:326563/55‑1 (MQ=255)
cccGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc > 1:326563/1‑55 (MQ=255)
ccGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc < 2:106335/139‑1 (MQ=255)
gcgGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTcatcat > 1:262975/1‑139 (MQ=255)
aGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa > 1:800012/1‑77 (MQ=255)
aGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa < 2:800012/77‑1 (MQ=255)
gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg < 2:262975/139‑1 (MQ=255)
|
CGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACG > NC_000913/4595395‑4595647
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
