Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,527	T→C	T109A (ACG→GCG)	lgoR ←	putative transcriptional activator for L‑galactonate catabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,527	0	T	C	100.0%	192.6 / NA	53	T109A (ACG→GCG)	lgoR	putative transcriptional activator for L‑galactonate catabolism
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/24); total (29/24)

CGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGT  >  NC_000913/4595419‑4595661

cgcgCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgcgc                                                                                                                  >  2:822123/1‑131 (MQ=255)

cgcgCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgcgc                                                                                                                  <  1:822123/131‑1 (MQ=255)

                          taACGGCCGAATTTTAAACGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTNCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca                                                                                >  2:465053/1‑139 (MQ=255)

                          taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGGCTGCTCa                                                                                    >  1:881137/1‑135 (MQ=255)

                          taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCa                                                                                    <  2:881137/135‑1 (MQ=255)

                          taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca                                                                                >  2:396923/1‑139 (MQ=255)

                                ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    <  2:768619/97‑1 (MQ=255)

                                ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    >  1:768619/1‑97 (MQ=255)

                                ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgt                                                                          <  2:472472/139‑1 (MQ=255)

                                ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgt                                                                          <  2:899601/139‑1 (MQ=255)

                                      tttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                    <  1:396923/139‑1 (MQ=255)

                                                             aaCCGGGCTGACACCCGCGGCCCGAACCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAg                                             >  1:787626/1‑139 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAAc                                                                                                                        >  1:852597/1‑63 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAAc                                                                                                                        <  2:852597/63‑1 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    <  2:294698/67‑1 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    >  1:294698/1‑67 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                          >  1:987055/1‑93 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                          <  2:987055/93‑1 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   >  1:384184/1‑100 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   <  2:384184/100‑1 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                              >  2:110578/1‑105 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                              <  1:110578/105‑1 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:770532/1‑139 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  2:1097999/1‑139 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:964738/1‑139 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:178532/1‑139 (MQ=255)

                                                              aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:357782/1‑139 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    >  1:294517/1‑63 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   >  1:1304245/1‑96 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   >  1:1335746/1‑96 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   <  2:1304245/96‑1 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   <  2:1335746/96‑1 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                        >  2:973407/1‑123 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                        <  1:973407/123‑1 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  1:1001906/1‑139 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  2:913271/1‑139 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  1:856097/1‑139 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  2:750194/1‑139 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTAGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  1:846481/1‑139 (MQ=255)

                                                                  ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCGGGGCGTAACTGgc                                                                                                                    <  2:294517/63‑1 (MQ=255)

                                                                           ccGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                               <  1:1097999/139‑1 (MQ=255)

                                                                             gcgGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGc                                                       >  2:59821/1‑113 (MQ=255)

                                                                             gcgGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGc                                                       <  1:59821/113‑1 (MQ=255)

                                                                                gCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                          <  2:964738/139‑1 (MQ=255)

                                                                                 cccGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTc                                                                                     <  2:886160/79‑1 (MQ=255)

                                                                                 cccGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                           <  1:347710/89‑1 (MQ=255)

                                                                                 cccGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                           >  2:347710/1‑89 (MQ=255)

                                                                                 cccGAGCCAGTTGCAGTTAAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTc                                                                                     >  1:886160/1‑79 (MQ=255)

                                                                                          gTTGCAGTTCAGAAAATGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  1:913271/139‑1 (MQ=255)

                                                                                          gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGTTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  2:846481/139‑1 (MQ=255)

                                                                                          gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  1:750194/139‑1 (MQ=255)

                                                                                          gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  2:856097/139‑1 (MQ=255)

                                                                                                        aaCGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGt  >  1:654112/1‑139 (MQ=255)

CGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGT  >  NC_000913/4595419‑4595661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A