Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,763,987 Δ1 bp coding (23/1488 nt) sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,9870G.100.0% 238.6 / NA 59coding (23/1488 nt)sufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (29/30);  total (29/30)

CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGAC  >  NC_000913/1763854‑1764113
                                                                                                                                     |                                                                                                                              
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTAGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt                                                                                                                          >  2:1256735/1‑139 (MQ=255)
 tCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTc                                                                                                                         <  2:1059801/139‑1 (MQ=255)
 tCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTc                                                                                                                         <  1:1378/139‑1 (MQ=255)
 tCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTc                                                                                                                         <  1:735885/139‑1 (MQ=255)
   aTTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAg                                                                                                                       >  2:1616160/1‑139 (MQ=255)
    ttACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGt                                                                                                                      >  2:12388/1‑139 (MQ=255)
     tACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCTAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCt                                                                                                                           <  2:999824/133‑1 (MQ=255)
     tACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCt                                                                                                                           >  1:999824/1‑133 (MQ=255)
          ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg                                                                                                                <  1:1616160/139‑1 (MQ=255)
               cGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCt                                                                                                                           >  1:842343/1‑123 (MQ=255)
               cGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCt                                                                                                                           <  2:842343/123‑1 (MQ=255)
               cGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACa                                                                                                           >  2:1232898/1‑139 (MQ=255)
                           caccacCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGctta                                                                                               <  1:1505663/139‑4 (MQ=255)
                                    ttCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCaaa                                                                                      >  2:646940/1‑139 (MQ=255)
                                      cGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGc                                                                                    >  2:290834/1‑139 (MQ=255)
                                          tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCt                                                                                                                           <  1:1404977/96‑1 (MQ=255)
                                          tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCt                                                                                                                           >  2:1404977/1‑96 (MQ=255)
                                            tCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                              >  1:1563470/1‑139 (MQ=255)
                                                cTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                                          >  1:1447384/1‑139 (MQ=255)
                                                       aGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                   <  1:1256735/139‑1 (MQ=255)
                                                             tCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                             >  1:293405/1‑139 (MQ=255)
                                                              cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                            <  2:293405/139‑1 (MQ=255)
                                                              cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                            <  1:290834/139‑1 (MQ=255)
                                                                      aaCTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa                                                    <  2:863720/139‑1 (MQ=255)
                                                                                  aaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAAt                                        <  2:1766949/139‑1 (MQ=255)
                                                                                     ccTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                        <  2:535549/104‑1 (MQ=255)
                                                                                     ccTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                        >  1:535549/1‑104 (MQ=255)
                                                                                          tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                <  2:1447384/139‑1 (MQ=255)
                                                                                            tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGctta                                                                                               <  2:85503/74‑4 (MQ=255)
                                                                                            tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTta                                                                                               >  1:85503/1‑74 (MQ=255)
                                                                                            tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                             >  2:1833132/1‑108 (MQ=255)
                                                                                            tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                             <  1:1833132/108‑1 (MQ=255)
                                                                                                    aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                        <  2:1039725/89‑1 (MQ=255)
                                                                                                    aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                        >  1:1039725/1‑89 (MQ=255)
                                                                                                      cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAg                                                                                                                       <  1:909846/40‑1 (MQ=255)
                                                                                                      cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAg                                                                                                                       >  2:909846/1‑40 (MQ=255)
                                                                                                      cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                >  1:1689921/1‑127 (MQ=255)
                                                                                                      cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                <  2:1689921/127‑1 (MQ=255)
                                                                                                      cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                    >  1:1605504/1‑139 (MQ=255)
                                                                                                        ggCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCtt                                                                                  >  2:1847134/1‑75 (MQ=255)
                                                                                                        ggCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCtt                                                                                  <  1:1847134/75‑1 (MQ=255)
                                                                                                         gccgccGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTa                                                                                                                  <  2:315211/42‑1 (MQ=255)
                                                                                                         gccgccGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTa                                                                                                                  >  1:315211/1‑42 (MQ=255)
                                                                                                         gccgccGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACga                                                                                                               <  1:257111/45‑1 (MQ=255)
                                                                                                         gccgccGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACga                                                                                                               >  2:257111/1‑45 (MQ=255)
                                                                                                           cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCac               >  1:129885/1‑139 (MQ=255)
                                                                                                                  ccAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCAcgaacgaa        <  2:129885/139‑1 (MQ=255)
                                                                                                                     ggTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATc     <  1:646940/139‑1 (MQ=255)
                                                                                                                        tttGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCAAAAGCTTTCGCCACAGCCACCTTCGTTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGAc  >  2:1150742/1‑139 (MQ=255)
                                                                                                                          tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                                          <  2:1470277/65‑1 (MQ=255)
                                                                                                                          tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                                          >  1:1470277/1‑65 (MQ=255)
                                                                                                                          tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                               <  2:897578/108‑1 (MQ=255)
                                                                                                                          tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                               >  1:897578/1‑108 (MQ=255)
                                                                                                                           gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                    >  2:1045092/1‑102 (MQ=255)
                                                                                                                           gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTGTAGGGTTGTGAAATTTGa                                    <  1:1045092/102‑1 (MQ=255)
                                                                                                                               tCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                        <  1:1409436/62‑1 (MQ=255)
                                                                                                                               tCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                        >  2:1409436/1‑62 (MQ=255)
                                                                                                                               tCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                  <  1:17860/100‑1 (MQ=255)
                                                                                                                               tCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                  >  2:17860/1‑100 (MQ=255)
                                                                                                                                     |                                                                                                                              
CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGAC  >  NC_000913/1763854‑1764113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A