Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
773 |
79.9 |
4112934 |
92.2% |
3792125 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,763,987 |
Δ1 bp |
coding (23/1488 nt) |
sufB ← |
component of SufBCD Fe‑S cluster assembly scaffold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,763,987 | 0 | G | . | 98.2%
| 222.5
/ ‑2.6
| 56 | coding (23/1488 nt) | sufB | component of SufBCD Fe‑S cluster assembly scaffold |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (24/31); total (24/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGT > NC_000913/1763854‑1764119
|
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt < 1:1232563/139‑1 (MQ=255)
aTTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAg > 1:1051186/1‑139 (MQ=255)
cTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTAc > 1:1549495/1‑139 (MQ=255)
ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg < 1:924658/139‑1 (MQ=255)
ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg > 2:1292723/1‑139 (MQ=255)
ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg < 1:1613796/139‑1 (MQ=255)
aTCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGCTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCg > 1:1472103/1‑139 (MQ=255)
tCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc < 2:1180855/139‑1 (MQ=255)
cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca < 2:25567/139‑1 (MQ=255)
ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc < 2:1051186/139‑1 (MQ=255)
ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc < 2:1472103/139‑1 (MQ=255)
ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc < 2:1549495/139‑1 (MQ=255)
aGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat < 1:1009103/139‑1 (MQ=255)
ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCATGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg < 1:839436/139‑1 (MQ=255)
ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATGCAGCGGGCCGCCGGTCCAGGTTTTGTCATCGTCAGTT‑CTTCAGTATTACGAGACATAGTACCGCTTATACCCTAAAGCTTTCGCCACAGCCACTTTCATTCTg < 1:502516/139‑1 (MQ=255)
gCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg > 1:1928272/1‑82 (MQ=255)
gCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg < 2:1928272/82‑1 (MQ=255)
tAACTGGGTGAATAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACAGCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCttt < 2:1946130/139‑1 (MQ=255)
ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCGCAAAGCTTTCGCCACAGCCACATTCATTCTg > 1:1336599/1‑127 (MQ=255)
ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa < 1:1691105/98‑1 (MQ=255)
ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa > 2:1691105/1‑98 (MQ=255)
ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACAGTCATTCTg < 2:1336599/127‑1 (MQ=255)
tGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTa > 1:1674856/1‑82 (MQ=255)
tGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTa < 2:1674856/82‑1 (MQ=255)
tGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCtt > 1:26386/1‑130 (MQ=255)
tGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCtt < 2:26386/130‑1 (MQ=255)
cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt > 1:841059/1‑53 (MQ=255)
cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt < 2:841059/53‑1 (MQ=255)
tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACAt < 1:403532/63‑1 (MQ=255)
tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACAt > 2:403532/1‑63 (MQ=255)
aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGAt > 1:787597/1‑139 (MQ=255)
ttCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTAc > 1:374849/1‑63 (MQ=255)
ttCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTAc < 2:374849/63‑1 (MQ=255)
ttCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat > 1:1162071/1‑130 (MQ=255)
ttCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat < 2:1162071/130‑1 (MQ=255)
cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg < 1:1292723/139‑1 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg < 2:1424286/47‑1 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg > 1:1424286/1‑47 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTAc < 1:608457/58‑1 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTAc > 2:608457/1‑58 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa < 2:1554280/107‑1 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa > 1:1554280/1‑107 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGaa > 1:170679/1‑117 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGaa < 2:170679/117‑1 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc > 1:502773/1‑139 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATATGATTAAGTcc > 2:1332390/1‑139 (MQ=255)
cGGGCCGCCGGTCCAGG‑TTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCGCAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCt > 1:1215654/1‑139 (MQ=255)
cgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacgaa < 1:1332390/139‑1 (MQ=255)
tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt > 1:1519075/1‑79 (MQ=255)
tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt < 2:1519075/79‑1 (MQ=255)
gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGCACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTc < 2:1787063/121‑1 (MQ=255)
gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGCACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTc > 1:1787063/1‑121 (MQ=255)
aCATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTcc < 2:1215654/139‑1 (MQ=255)
aTCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGt < 2:787597/139‑1 (MQ=255)
tCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacga > 2:1749806/1‑121 (MQ=255)
tCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacga < 1:1749806/121‑1 (MQ=255)
|
CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGT > NC_000913/1763854‑1764119
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
