Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,595,227 T→C Q211Q (CAA→CAG)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,595,2270TC100.0% 164.5 / NA 47Q211Q (CAA→CAG) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/21);  total (26/21)

GCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCA  >  NC_000913/2595093‑2595355
                                                                                                                                      |                                                                                                                                
gCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGcc                                                                                                                              <  2:196048/139‑1 (MQ=255)
gCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGcc                                                                                                                              >  2:1102986/1‑139 (MQ=255)
gCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGcc                                                                                                                              >  1:453407/1‑139 (MQ=255)
gCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGcc                                                                                                                              >  1:471580/1‑139 (MQ=255)
       cATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCaa                                                                                                                       >  2:1617711/1‑139 (MQ=255)
                    cAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACcg                                                                                                          >  1:688231/1‑139 (MQ=255)
                      aGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACcgcg                                                                                                        <  1:334978/139‑1 (MQ=255)
                         cATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGccc                                                                                                     >  1:440099/1‑139 (MQ=255)
                             cGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgc                                                                                                 >  2:1490978/1‑139 (MQ=255)
                               gcgcAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACgcgc                                                                                               >  2:1648364/1‑139 (MQ=255)
                                cgcAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCa                                                                                              >  1:1751696/1‑139 (MQ=255)
                                            aaaCTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCt                                                                                  >  1:1173244/1‑139 (MQ=255)
                                                  ctcGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACg                                                                            >  2:149696/1‑139 (MQ=255)
                                                    cGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                                                                <  1:801412/117‑1 (MQ=255)
                                                    cGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                                                                >  2:801412/1‑117 (MQ=255)
                                                       ccGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcgg                                                                       <  1:1490978/139‑1 (MQ=255)
                                                          cAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTAcc                                                                                                           <  1:599223/100‑1 (MQ=255)
                                                          cAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTAcc                                                                                                           >  2:599223/1‑100 (MQ=255)
                                                            aaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                                  <  2:471580/139‑1 (MQ=255)
                                                                   ccAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCAt                                                           <  1:634193/139‑1 (MQ=255)
                                                                      gTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAg                                                        <  2:1751696/139‑1 (MQ=255)
                                                                      gTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAg                                                        <  2:425430/139‑1 (MQ=255)
                                                                       tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc                                                       <  1:467800/139‑1 (MQ=255)
                                                                            ccGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                                 <  1:670825/76‑1 (MQ=255)
                                                                            ccGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                                 >  2:670825/1‑76 (MQ=255)
                                                                                tGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc                                                       >  1:776799/1‑130 (MQ=255)
                                                                                tGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc                                                       <  2:776799/130‑1 (MQ=255)
                                                                                 gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                                 <  1:550836/71‑1 (MQ=255)
                                                                                 gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                                 >  2:550836/1‑71 (MQ=255)
                                                                                 gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcg                                                                        >  2:101149/1‑112 (MQ=255)
                                                                                 gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcg                                                                        <  1:101149/112‑1 (MQ=255)
                                                                                 gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAaga                                             >  2:1805855/1‑139 (MQ=255)
                                                                                    gCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGtt                                          >  2:1497487/1‑139 (MQ=255)
                                                                                      ttttGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGc                                        <  1:848139/139‑1 (MQ=255)
                                                                                        ttGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCtg                                      <  1:1805855/139‑1 (MQ=255)
                                                                                                          tCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTgc                    >  2:1540094/1‑139 (MQ=255)
                                                                                                           cGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTgcg                   <  2:440099/139‑1 (MQ=255)
                                                                                                            gCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGcc                                                                          <  2:1869281/83‑1 (MQ=255)
                                                                                                            gCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGcc                                                                          >  1:1869281/1‑83 (MQ=255)
                                                                                                             ctctGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGcc                 >  2:1516269/1‑139 (MQ=255)
                                                                                                              tctGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCg                                                                                                                  >  2:1681721/1‑41 (MQ=255)
                                                                                                              tctGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCg                                                                                                                  <  1:1681721/41‑1 (MQ=255)
                                                                                                                         tACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTa     <  2:688231/139‑1 (MQ=255)
                                                                                                                            gagaAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACca  >  2:1561637/1‑139 (MQ=255)
                                                                                                                                 aTGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGcc                                                                          >  1:895117/1‑62 (MQ=255)
                                                                                                                                 aTGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGcc                                                                          <  2:895117/62‑1 (MQ=255)
                                                                                                                                   gAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTa                                                         >  1:1195506/1‑77 (MQ=255)
                                                                                                                                      |                                                                                                                                
GCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCA  >  NC_000913/2595093‑2595355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A