Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,595,227	T→C	Q211Q (CAA→CAG)	tmcA ←	elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,595,227	0	T	C	100.0%	171.0 / NA	49	Q211Q (CAA→CAG)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/26); total (23/26)

GGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTA  >  NC_000913/2595092‑2595352

ggCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGCGAAATGAGCTGc                                                                                                                            <  1:1007103/139‑1 (MQ=255)

        cATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCaa                                                                                                                    <  2:1349937/139‑1 (MQ=255)

                     cAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACcg                                                                                                       >  2:1022355/1‑139 (MQ=255)

                     cAAGGCATCCGGCGCAAGAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGTGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACcg                                                                                                       >  1:1181214/1‑139 (MQ=255)

                               ggCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                                                             <  1:960972/139‑1 (MQ=255)

                                   cAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGc                                                                                         >  2:944159/1‑139 (MQ=255)

                                           cGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGTCGTTACCg                                                                                 >  2:835125/1‑139 (MQ=255)

                                            gAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                              <  1:1669390/109‑1 (MQ=255)

                                            gAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                              >  2:1669390/1‑109 (MQ=255)

                                             aaaCTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgc                                                                                              >  1:485422/1‑124 (MQ=255)

                                             aaaCTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgc                                                                                              <  2:485422/124‑1 (MQ=255)

                                              aaCTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTg                                                                              >  2:1983574/1‑139 (MQ=255)

                                                cTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGcc                                                                            >  2:537374/1‑139 (MQ=255)

                                                   ctcGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACg                                                                         <  1:492171/139‑1 (MQ=255)

                                                   ctcGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACAGCGCCCACGCGCAGCCGTTACCGCTGCCACg                                                                         >  2:737585/1‑139 (MQ=255)

                                                           cAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcggcgg                                                                 >  2:284751/1‑139 (MQ=255)

                                                           cAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcggcgg                                                                 >  1:1256133/1‑139 (MQ=255)

                                                                  tgCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTc                                                          >  2:1334248/1‑139 (MQ=255)

                                                                        tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcggcg                                                                  >  1:1184044/1‑125 (MQ=255)

                                                                        tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCcggcg                                                                  <  2:1184044/125‑1 (MQ=255)

                                                                        tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc                                                    <  2:1973509/139‑1 (MQ=255)

                                                                                ttGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTAcc                                                                                  >  1:1721416/1‑101 (MQ=255)

                                                                                ttGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTAcc                                                                                  <  2:1721416/101‑1 (MQ=255)

                                                                                 tGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAag                                           >  2:325433/1‑139 (MQ=255)

                                                                                 tGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAag                                           <  1:944159/139‑1 (MQ=255)

                                                                                    cGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGt                                        <  2:1181214/139‑1 (MQ=255)

                                                                                    cGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGt                                        >  1:666451/1‑139 (MQ=255)

                                                                                        tttGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCt                                    <  1:2038211/139‑1 (MQ=255)

                                                                                          tGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCGGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCtgt                                  <  1:1022355/139‑1 (MQ=255)

                                                                                          tGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGc                                     >  2:393934/1‑136 (MQ=255)

                                                                                          tGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGc                                     <  1:393934/136‑1 (MQ=255)

                                                                                                   ggTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCtgt                                  <  1:569476/130‑1 (MQ=255)

                                                                                                   ggTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCtgt                                  >  2:569476/1‑130 (MQ=255)

                                                                                                   ggTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGt                         <  1:737585/139‑1 (MQ=255)

                                                                                                       aCAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTtgtg                     <  1:537374/139‑1 (MQ=255)

                                                                                                        cAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGTCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTgtgg                    <  1:835125/139‑1 (MQ=255)

                                                                                                           tCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGcc                                                                            <  1:583456/80‑1 (MQ=255)

                                                                                                           tCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGcc                                                                            >  2:583456/1‑80 (MQ=255)

                                                                                                            cGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTgcg                <  1:325433/139‑1 (MQ=255)

                                                                                                               tctGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGt                                        >  2:233828/1‑112 (MQ=255)

                                                                                                               tctGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGt                                        <  1:233828/112‑1 (MQ=255)

                                                                                                                   cccGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTcg         <  1:1983574/139‑1 (MQ=255)

                                                                                                                       cAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                               >  2:611749/1‑81 (MQ=255)

                                                                                                                       cAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                               <  1:611749/81‑1 (MQ=255)

                                                                                                                          tACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTa  <  2:666451/139‑1 (MQ=255)

                                                                                                                             gagaAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                               <  2:1466744/75‑1 (MQ=255)

                                                                                                                             gagaAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                               >  1:1466744/1‑75 (MQ=255)

                                                                                                                               gaAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACACCCGGCGGCATGGTCATTAg                                                     >  1:215787/1‑83 (MQ=255)

                                                                                                                               gaAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACACCCGGCGGCATGGTCATTAg                                                     <  2:215787/83‑1 (MQ=255)

GGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTA  >  NC_000913/2595092‑2595352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A