Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,800,475 G→A *110* (TAG→TAA)  ygaM → putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,4750GA100.0% 205.6 / NA 63*110* (TAG→TAA) ygaMputative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/35);  total (28/35)

GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATA  >  NC_000913/2800344‑2800610
                                                                                                                                   |                                                                                                                                        
gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                                   >  2:1293125/1‑139 (MQ=255)
  ccAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt                                                                                                                                 <  2:1613729/139‑1 (MQ=255)
                       cGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTg                                                                                                            <  2:673586/139‑1 (MQ=255)
                                cgcgGATTCTTTTGTCCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAg                                                                                                  >  1:1253723/1‑139 (MQ=255)
                                   ggATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCt                                                                                               <  1:1819207/139‑1 (MQ=255)
                                          tttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCg                                                                                        <  1:1293125/139‑1 (MQ=255)
                                          tttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCg                                                                                        <  2:1882218/139‑1 (MQ=255)
                                              ttCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGt                                                                                    <  2:1501995/139‑1 (MQ=255)
                                                  tGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGa                                                                                                                               >  1:617762/1‑93 (MQ=255)
                                                  tGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGa                                                                                                                               <  2:617762/93‑1 (MQ=255)
                                                                    cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                              >  1:1140969/1‑139 (MQ=255)
                                                                         gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    <  1:1452376/96‑1 (MQ=255)
                                                                         gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    >  2:1452376/1‑96 (MQ=255)
                                                                         gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaaa                                                         >  1:1347872/1‑139 (MQ=255)
                                                                             agcagcTGCGGTAGGTATTTTTATCGGTGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                                     >  2:1058095/1‑139 (MQ=255)
                                                                              gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcccc                                                    <  2:87182/139‑1 (MQ=255)
                                                                                 gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATCAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCAC‑AAAATCCCCCTAc                                                <  2:1140969/139‑1 (MQ=255)
                                                                                    gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    <  1:451622/85‑1 (MQ=255)
                                                                                    gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    >  2:451622/1‑85 (MQ=255)
                                                                                     cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcccc                                             >  2:462772/1‑139 (MQ=255)
                                                                                        taggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTc                                          <  2:1620942/139‑1 (MQ=255)
                                                                                          ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCtt                                                                                                              <  1:560164/70‑1 (MQ=255)
                                                                                          ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCtt                                                                                                              >  2:560164/1‑70 (MQ=255)
                                                                                           gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                          >  2:1547428/1‑120 (MQ=255)
                                                                                           gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                          <  1:1547428/120‑1 (MQ=255)
                                                                                               ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               <  1:25807/127‑1 (MQ=255)
                                                                                               ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               >  2:25807/1‑127 (MQ=255)
                                                                                               ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                   >  2:425254/1‑139 (MQ=255)
                                                                                                  tATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCatat                                <  1:462772/139‑1 (MQ=255)
                                                                                                   aTCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                               >  1:1436561/1‑139 (MQ=255)
                                                                                                     cGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGcc                                                                                                <  1:1632743/72‑1 (MQ=255)
                                                                                                     cGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGcc                                                                                                >  2:1632743/1‑72 (MQ=255)
                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                            >  2:432919/1‑139 (MQ=255)
                                                                                                        cgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                          >  1:611798/1‑139 (MQ=255)
                                                                                                                 aaGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGa                 >  2:1774956/1‑139 (MQ=255)
                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCCTATCCAGGGTAATTTCGAc                <  2:1253723/139‑1 (MQ=255)
                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                >  1:554648/1‑139 (MQ=255)
                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                >  1:1224691/1‑139 (MQ=255)
                                                                                                                   gCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAcc               <  2:1436561/139‑1 (MQ=255)
                                                                                                                      tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt            <  1:1058095/139‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCttt                                                                              >  2:962824/1‑72 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCttt                                                                              <  1:962824/72‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                           >  1:673049/1‑91 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                           <  2:673049/91‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               <  2:168766/103‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               >  1:168766/1‑103 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                           >  2:172382/1‑107 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                           <  1:172382/107‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                >  2:1425055/1‑134 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                <  1:1425055/134‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa           >  1:947573/1‑139 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa           >  1:809602/1‑139 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  1:302632/139‑1 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  2:947573/139‑1 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  1:425254/139‑1 (MQ=255)
                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  2:850290/139‑1 (MQ=255)
                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg                                       <  1:1854377/109‑1 (MQ=255)
                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg                                       >  2:1854377/1‑109 (MQ=255)
                                                                                                                          cAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt        <  2:611798/139‑1 (MQ=255)
                                                                                                                            aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCTACCACTATTTGc      <  1:432919/139‑1 (MQ=255)
                                                                                                                            aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc      <  2:809602/139‑1 (MQ=255)
                                                                                                                             aTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt     <  1:1774956/139‑1 (MQ=255)
                                                                                                                                gTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtata  <  2:554648/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                        
GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATA  >  NC_000913/2800344‑2800610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A