Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,800,475	G→A	110 (TAG→TAA)	ygaM →	putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,800,475	0	G	A	100.0%	134.3 / NA	42	110 (TAG→TAA)	ygaM	putative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/23); total (19/23)

GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC  >  NC_000913/2800344‑2800606

gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                              >  2:151211/1‑139 (MQ=255)

          ccGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGAtaataa                                                                                                                    <  1:971696/139‑1 (MQ=255)

                                cgcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaa                                                                                                                               >  2:926395/1‑106 (MQ=255)

                                cgcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaa                                                                                                                               <  1:926395/106‑1 (MQ=255)

                                                        aCCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGc                                                                      >  2:303319/1‑139 (MQ=255)

                                                                   gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                           >  1:398294/1‑139 (MQ=255)

                                                                   gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                           >  2:290638/1‑139 (MQ=255)

                                                                        ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                      >  1:816368/1‑139 (MQ=255)

                                                                              gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcccc                                                <  2:473495/139‑1 (MQ=255)

                                                                               cagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccccc                                               <  1:290638/139‑1 (MQ=255)

                                                                                         aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     <  2:816368/139‑1 (MQ=255)

                                                                                         aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     <  2:107146/139‑1 (MQ=255)

                                                                                                  tATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCatat                            >  1:43027/1‑139 (MQ=255)

                                                                                                   aTCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                           <  1:303319/139‑1 (MQ=255)

                                                                                                   aTCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                           <  2:398294/139‑1 (MQ=255)

                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     >  1:925847/1‑126 (MQ=255)

                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     <  2:925847/126‑1 (MQ=255)

                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                        >  2:847628/1‑139 (MQ=255)

                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                        >  2:521249/1‑139 (MQ=255)

                                                                                                             tGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAgagcgtga                 <  1:151211/139‑8 (MQ=255)

                                                                                                               ttAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTc               >  2:654774/1‑139 (MQ=255)

                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc            <  1:847628/139‑1 (MQ=255)

                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc            <  1:941629/139‑1 (MQ=255)

                                                                                                                  aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc            <  1:521249/139‑1 (MQ=255)

                                                                                                                      tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt        <  1:654774/139‑1 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATAcg                                                                                                               >  1:658311/1‑35 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATAcg                                                                                                               <  2:658311/35‑1 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCTCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     <  1:659742/109‑1 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     >  2:659742/1‑109 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt        >  1:1003362/1‑138 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt        <  2:1003362/138‑1 (MQ=255)

                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa       >  1:426914/1‑139 (MQ=255)

                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  2:426914/139‑1 (MQ=255)

                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  2:43027/139‑1 (MQ=255)

                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTAGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  1:610888/139‑1 (MQ=255)

                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCAc         <  2:233371/135‑1 (MQ=255)

                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCAc         >  1:233371/1‑135 (MQ=255)

                                                                                                                         gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAtt     >  1:543644/1‑139 (MQ=255)

                                                                                                                              tCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt    >  1:574020/1‑135 (MQ=255)

                                                                                                                              tCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt    <  2:574020/135‑1 (MQ=255)

                                                                                                                              tCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc  <  1:121834/137‑1 (MQ=255)

                                                                                                                              tCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc  >  2:121834/1‑137 (MQ=255)

GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC  >  NC_000913/2800344‑2800606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A