Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,966	A→G	T16A (ACC→GCC)	mhpF →	acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,966	0	A	G	100.0%	175.7 / NA	49	T16A (ACC→GCC)	mhpF	acetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/22); total (27/22)

GGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372837‑373099

gggCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtga                                                                                                                              >  2:990107/1‑139 (MQ=255)

gggCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtga                                                                                                                              >  1:1322959/1‑139 (MQ=255)

                            ggCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTTCGTCACGGTCAGCa                                                                                                  >  1:245258/1‑139 (MQ=255)

                             gCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCAt                                                                                                 <  1:990107/139‑1 (MQ=255)

                                tAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTg                                                                                              >  1:32357/1‑139 (MQ=255)

                                            ctgcGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  >  2:668057/1‑139 (MQ=255)

                                                 aCATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGCGATGGCGGTGATGGt                                                                             >  1:287156/1‑139 (MQ=255)

                                                    ttttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                          <  1:226886/139‑1 (MQ=255)

                                                               aGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAg                                                                                                    <  1:1270727/102‑1 (MQ=255)

                                                               aGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAg                                                                                                    >  2:1270727/1‑102 (MQ=255)

                                                                 ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCAt                                                                                                 <  2:86188/103‑1 (MQ=255)

                                                                 ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCAt                                                                                                 >  1:86188/1‑103 (MQ=255)

                                                                      aaaGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCGGTCCGa                                                        >  2:517185/1‑139 (MQ=255)

                                                                           aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGAt                                                                                                                                  >  1:1295460/1‑60 (MQ=255)

                                                                           aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGAt                                                                                                                                  <  2:1295460/60‑1 (MQ=255)

                                                                            aGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  >  2:1509053/1‑107 (MQ=255)

                                                                            aGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  <  1:1509053/107‑1 (MQ=255)

                                                                             gtctgtctTGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCAt                                                                                                 <  1:817143/91‑1 (MQ=255)

                                                                             gTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCAt                                                                                                 >  2:817143/1‑91 (MQ=255)

                                                                                tGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            >  2:1164772/1‑109 (MQ=255)

                                                                                tGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            <  1:1164772/109‑1 (MQ=255)

                                                                                     tGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcgcgcgc                                         >  1:1044/1‑139 (MQ=255)

                                                                                            gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              >  2:196548/1‑127 (MQ=255)

                                                                                            gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              >  2:277571/1‑127 (MQ=255)

                                                                                            gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              <  1:196548/127‑1 (MQ=255)

                                                                                            gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              <  1:277571/127‑1 (MQ=255)

                                                                                                 aaGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAg                                                                                                    <  1:1183530/68‑1 (MQ=255)

                                                                                                 aaGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAg                                                                                                    >  2:1183530/1‑68 (MQ=255)

                                                                                                   gTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           >  2:251875/1‑59 (MQ=255)

                                                                                                   gTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           <  1:251875/59‑1 (MQ=255)

                                                                                                      gCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCg                        >  1:1181330/1‑139 (MQ=255)

                                                                                                       ccATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATgg                                                                                       >  2:1307083/1‑75 (MQ=255)

                                                                                                       ccATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATgg                                                                                       <  1:1307083/75‑1 (MQ=255)

                                                                                                          ttATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    <  2:32357/139‑1 (MQ=255)

                                                                                                             tCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCGGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCcaccac                 <  1:517185/139‑1 (MQ=255)

                                                                                                             tCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCcaccac                 <  2:1177596/139‑1 (MQ=255)

                                                                                                                  tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATg            >  2:1137023/1‑139 (MQ=255)

                                                                                                                      gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTcc                                                          >  2:578495/1‑89 (MQ=255)

                                                                                                                      gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTcc                                                          <  1:578495/89‑1 (MQ=255)

                                                                                                                       cAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAg                                                                                                    <  2:1418244/46‑1 (MQ=255)

                                                                                                                       cAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAg                                                                                                    >  1:1418244/1‑46 (MQ=255)

                                                                                                                       cAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAggg       <  1:668057/139‑1 (MQ=255)

                                                                                                                           aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCAtt                                                                      <  2:1528027/72‑1 (MQ=255)

                                                                                                                           aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCAtt                                                                      >  1:1528027/1‑72 (MQ=255)

                                                                                                                           aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    <  1:997184/122‑1 (MQ=255)

                                                                                                                           aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGa   >  2:287194/1‑139 (MQ=255)

                                                                                                                           aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGa   >  1:670146/1‑139 (MQ=255)

                                                                                                                           aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCACGCGCCAGACGTATGGGCGTCGccac                    >  2:997184/1‑122 (MQ=255)

                                                                                                                            ttGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAt  <  2:717371/139‑1 (MQ=255)

GGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372837‑373099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A