Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 372,966 A→G T16A (ACC→GCC)  mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913372,9660AG100.0% 234.8 / NA 65T16A (ACC→GCC) mhpFacetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

TGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372830‑373099
                                                                                                                                        |                                                                                                                                     
tGAATGCGGGCGATCGTTTTGAAGGCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGcc                                                                                                                                     >  2:1121550/1‑139 (MQ=255)
    tGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATc                                                                                                                                 >  2:515502/1‑139 (MQ=255)
    tGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATc                                                                                                                                 >  2:1807798/1‑139 (MQ=255)
        ggCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtgat                                                                                                                             <  1:1121550/139‑1 (MQ=255)
         gCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtgatg                                                                                                                            >  2:1730640/1‑139 (MQ=255)
                        cccATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGt                                                                                                             <  1:1807798/139‑1 (MQ=255)
                         ccATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTc                                                                                                            <  1:1730640/139‑1 (MQ=255)
                            tatTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACg                                                                                                         <  1:515502/139‑1 (MQ=255)
                               tGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  1:323774/139‑1 (MQ=255)
                                   ggCATAGGTTCGGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCa                                                                                                  >  1:538763/1‑139 (MQ=255)
                                   ggCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCa                                                                                                  >  2:486373/1‑139 (MQ=255)
                                      aTAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCt                                                                                               >  2:1195682/1‑139 (MQ=255)
                                       tAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTg                                                                                              >  1:1525843/1‑139 (MQ=255)
                                        aGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      >  1:933900/1‑130 (MQ=255)
                                        aGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  2:933900/130‑1 (MQ=255)
                                        aGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTgg                                                                                             >  1:174318/1‑139 (MQ=255)
                                            tcggTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         <  2:538763/136‑1 (MQ=255)
                                             cAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATg                                                                                        <  2:1525843/139‑1 (MQ=255)
                                                         cATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATgg                                                                                       >  2:783291/1‑128 (MQ=255)
                                                         cATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATgg                                                                                       <  1:783291/128‑1 (MQ=255)
                                                         cATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            <  2:455869/139‑1 (MQ=255)
                                                             ttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      >  1:200597/1‑109 (MQ=255)
                                                             ttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  2:200597/109‑1 (MQ=255)
                                                                   cgcAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  >  2:137467/1‑139 (MQ=255)
                                                                   cgcAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  >  2:124710/1‑139 (MQ=255)
                                                                       gCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCa                                                              >  2:1039445/1‑139 (MQ=255)
                                                                       gCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCa                                                              <  1:1039445/139‑1 (MQ=255)
                                                                        ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                               >  2:363489/1‑73 (MQ=255)
                                                                        ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                               <  1:363489/73‑1 (MQ=255)
                                                                        ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGga                                                                                            <  1:1076328/108‑1 (MQ=255)
                                                                        ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGga                                                                                            >  2:1076328/1‑108 (MQ=255)
                                                                        ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                             >  1:1645083/1‑139 (MQ=255)
                                                                        ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                             >  1:1472511/1‑139 (MQ=255)
                                                                           cAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTcc                                                          >  2:524171/1‑139 (MQ=255)
                                                                            aaaaGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCg                                                         <  1:246220/139‑1 (MQ=255)
                                                                                  aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           >  1:697858/1‑83 (MQ=255)
                                                                                  aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           <  2:697858/83‑1 (MQ=255)
                                                                                  aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTc                                                   >  1:794966/1‑139 (MQ=255)
                                                                                     tCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  >  1:223098/1‑105 (MQ=255)
                                                                                     tCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  <  2:223098/105‑1 (MQ=255)
                                                                                       tGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  <  1:1485348/119‑1 (MQ=255)
                                                                                       tGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  >  2:1485348/1‑119 (MQ=255)
                                                                                         tCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcgcg                                            <  2:174318/139‑1 (MQ=255)
                                                                                                   gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         <  2:1465969/84‑1 (MQ=255)
                                                                                                   gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         >  1:1465969/1‑84 (MQ=255)
                                                                                                        aaGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATggg                             >  2:1564577/1‑139 (MQ=255)
                                                                                                                aTTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcca                     <  1:124710/139‑1 (MQ=255)
                                                                                                                 ttATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    <  1:1133409/139‑1 (MQ=255)
                                                                                                                       gTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              >  2:1566746/1‑107 (MQ=255)
                                                                                                                       gTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              <  1:1566746/107‑1 (MQ=255)
                                                                                                                         tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATg            <  1:1195682/139‑1 (MQ=255)
                                                                                                                           tGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         <  1:45679/60‑1 (MQ=255)
                                                                                                                           tGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         >  2:45679/1‑60 (MQ=255)
                                                                                                                            gtcAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCCCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAg         >  2:733202/3‑139 (MQ=255)
                                                                                                                            ggCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAg         >  1:150711/1‑139 (MQ=255)
                                                                                                                             gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           >  2:1683395/1‑40 (MQ=255)
                                                                                                                             gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           <  1:1683395/40‑1 (MQ=255)
                                                                                                                             gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcca                     <  1:376937/126‑1 (MQ=255)
                                                                                                                             gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcca                     >  2:376937/1‑126 (MQ=255)
                                                                                                                             gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAgg        <  1:486373/139‑1 (MQ=255)
                                                                                                                                aCATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                          <  2:684287/70‑1 (MQ=255)
                                                                                                                                aCATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                          >  1:684287/1‑70 (MQ=255)
                                                                                                                                  aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            <  2:1509470/66‑1 (MQ=255)
                                                                                                                                  aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            >  1:1509470/1‑66 (MQ=255)
                                                                                                                                   ttGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAt  <  2:794966/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372830‑373099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A