Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,966	A→G	T16A (ACC→GCC)	mhpF →	acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,966	0	A	G	100.0%	148.3 / NA	42	T16A (ACC→GCC)	mhpF	acetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/21); total (21/21)

TGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372834‑373099

tGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGGGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATc                                                                                                                                 >  2:692395/1‑139 (MQ=255)

                      cATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           >  1:1115114/1‑139 (MQ=255)

                           tGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  2:581969/139‑1 (MQ=255)

                            gAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCa                                                                                                     <  2:1115114/139‑1 (MQ=255)

                              aGGCATAGGTTCAGTTGCTGAGACATTTTCAAGCGCAGCCCCAAAAGAAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGc                                                                                                   >  1:541040/1‑139 (MQ=255)

                               ggCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCa                                                                                                  >  2:1024167/1‑139 (MQ=255)

                                       ttCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGgaga                                                                                          >  2:608768/1‑139 (MQ=255)

                                                    aCATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGt                                                                             >  2:338765/1‑139 (MQ=255)

                                                       ttttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                          >  1:471563/1‑139 (MQ=255)

                                                          tCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCAt                                                                       <  1:487301/139‑1 (MQ=255)

                                                           cAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCAtt                                                                      <  2:471563/139‑1 (MQ=255)

                                                               cgcAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTTAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGt                                                                             >  1:1092327/1‑128 (MQ=255)

                                                               cgcAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTTAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGt                                                                             <  2:1092327/128‑1 (MQ=255)

                                                                 cAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCt                                                                <  1:338765/139‑1 (MQ=255)

                                                                    ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                               >  2:994269/1‑73 (MQ=255)

                                                                    ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                               <  1:994269/73‑1 (MQ=255)

                                                                    ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                             >  2:488525/1‑139 (MQ=255)

                                                                    ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                             >  2:288813/1‑139 (MQ=255)

                                                                        aaaaGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCg                                                         <  1:608768/139‑1 (MQ=255)

                                                                              aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTc                                                   >  2:972900/1‑139 (MQ=255)

                                                                                        tGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcgcgcgc                                         <  1:288813/139‑1 (MQ=255)

                                                                                          aGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  >  1:950847/1‑96 (MQ=255)

                                                                                          aGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  <  2:950847/96‑1 (MQ=255)

                                                                                             aaGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgc                                               <  1:787655/128‑1 (MQ=255)

                                                                                             aaGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgc                                               >  2:787655/1‑128 (MQ=255)

                                                                                               gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      >  2:1104968/1‑71 (MQ=255)

                                                                                               gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  1:1104968/71‑1 (MQ=255)

                                                                                               gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            >  2:205276/1‑97 (MQ=255)

                                                                                               gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            <  1:205276/97‑1 (MQ=255)

                                                                                               gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGt                                  >  2:1105643/1‑139 (MQ=255)

                                                                                                          ccATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGc                       <  1:1105643/139‑1 (MQ=255)

                                                                                                          ccATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGAT‑CTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcc                      >  1:951962/1‑139 (MQ=255)

                                                                                                             ttATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    <  1:1024167/139‑1 (MQ=255)

                                                                                                               aTCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCt                                                                <  2:569976/93‑1 (MQ=255)

                                                                                                               aTCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCt                                                                >  1:569976/1‑93 (MQ=255)

                                                                                                                     tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATg            <  1:488525/139‑1 (MQ=255)

                                                                                                                       tGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGaa          >  2:463122/1‑139 (MQ=255)

                                                                                                                       tGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGaa          <  1:1036275/139‑1 (MQ=255)

                                                                                                                         gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    <  1:266148/127‑1 (MQ=255)

                                                                                                                         gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    >  2:266148/1‑127 (MQ=255)

                                                                                                                              aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGAT‑CTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAt  <  2:951962/139‑1 (MQ=255)

                                                                                                                               ttGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAt  <  1:972900/139‑1 (MQ=255)

                                                                                                                               ttGGTGCCCATCTGATGATTAAAATTTTGCTTCTCGGTCAGCTTCTGGAGTTTGCCGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGCCGTATGGGCGTCGCCACCACCCATGCAGTGGTTAt  <  2:541040/139‑1 (MQ=255)

TGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372834‑373099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A