Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,322,601 |
C→T |
A116T (GCC→ACC) |
trpE ← |
component I of anthranilate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,322,601 | 0 | C | T | 100.0%
| 213.3
/ NA
| 66 | A116T (GCC→ACC) | trpE | component I of anthranilate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (32/34); total (32/34) |
AAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAG > NC_000913/1322465‑1322730
|
aaaTCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGgtgt < 1:360766/139‑1 (MQ=255)
aaaTCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATcc > 2:300103/1‑139 (MQ=255)
tCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc < 2:317185/139‑1 (MQ=255)
ccGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag > 2:1491647/1‑139 (MQ=255)
ccGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag > 2:1487851/1‑139 (MQ=255)
gCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGGTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTg > 2:224396/1‑139 (MQ=255)
ccACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTgg > 1:765072/1‑139 (MQ=255)
caAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACt < 1:627186/139‑1 (MQ=255)
caAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACt < 1:902037/139‑1 (MQ=255)
agagAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGGTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg < 1:224396/139‑1 (MQ=255)
aaCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAgc > 2:398362/1‑139 (MQ=255)
aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag < 2:1312664/118‑1 (MQ=255)
aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag > 1:1312664/1‑118 (MQ=255)
aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg < 1:242171/134‑1 (MQ=255)
aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg > 2:242171/1‑134 (MQ=255)
aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAgcg < 2:1279283/139‑1 (MQ=255)
aGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgca < 1:889425/139‑1 (MQ=255)
gccgccGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag < 1:467173/114‑1 (MQ=255)
gccgccGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag > 2:467173/1‑114 (MQ=255)
gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAg < 2:526512/122‑1 (MQ=255)
gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAg > 1:526512/1‑122 (MQ=255)
gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgcagca < 2:952934/137‑1 (MQ=255)
gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGcaca > 1:734836/1‑139 (MQ=255)
gccgAAGAACATGGCTTCTCGTTCTTCCCTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTAAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgcagca > 1:952934/1‑137 (MQ=255)
ccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCacac > 2:493273/1‑139 (MQ=255)
tGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTgg < 2:231077/139‑1 (MQ=255)
tGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTgg < 2:951330/139‑1 (MQ=255)
ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc < 1:1497537/97‑1 (MQ=255)
ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc > 2:1497537/1‑97 (MQ=255)
ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGt > 2:962617/1‑139 (MQ=255)
ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGt > 2:260254/1‑139 (MQ=255)
ctcGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTg > 2:1273443/1‑139 (MQ=255)
tcGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGt < 1:1491647/139‑1 (MQ=255)
gTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc < 2:1527556/89‑1 (MQ=255)
gTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc > 1:1527556/1‑89 (MQ=255)
cGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCcaca > 1:701701/1‑139 (MQ=255)
aTTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGTGGGGAAGCGCAGCACACgg < 1:1022602/106‑1 (MQ=255)
aTTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACgg > 2:1022602/1‑106 (MQ=255)
ttCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcag > 2:117074/1‑139 (MQ=255)
aaCAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcaggca < 1:300103/139‑1 (MQ=255)
aGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAggg > 2:439636/1‑139 (MQ=255)
aTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCg < 1:1273443/139‑1 (MQ=255)
aTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCg < 2:765072/139‑1 (MQ=255)
aTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCg < 1:493273/139‑1 (MQ=255)
ttCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCa > 2:105219/1‑57 (MQ=255)
ttCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCa < 1:105219/57‑1 (MQ=255)
tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGAc < 1:394948/69‑1 (MQ=255)
tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGAc > 2:394948/1‑69 (MQ=255)
tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGc < 2:572769/96‑1 (MQ=255)
tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGc > 1:572769/1‑96 (MQ=255)
cTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag < 1:1433294/63‑1 (MQ=255)
cTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag > 2:1433294/1‑63 (MQ=255)
cTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTa < 1:1487851/139‑1 (MQ=255)
gCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCt > 1:62469/1‑50 (MQ=255)
gCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCt < 2:62469/50‑1 (MQ=255)
aaaCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag > 1:871065/1‑56 (MQ=255)
aaaCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag < 2:871065/56‑1 (MQ=255)
aGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCagg < 1:260254/139‑1 (MQ=255)
gCGTCAAAAACCGAAAGGGAGCATAAGC‑GGTGTCTTCATCCAGCAGTGGACTGACAgggggg > 1:916565/1‑62 (MQ=255)
gCGTCAAAAACCGAAAGGGAGCATAAGC‑GGTGTCTTCATCCAGCAGTGGACTGACAgggggg < 2:916565/62‑1 (MQ=38)
gTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg > 2:681934/1‑61 (MQ=255)
gTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg < 1:681934/61‑1 (MQ=255)
tCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCca < 2:875552/100‑1 (MQ=255)
tCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCca > 1:875552/1‑100 (MQ=255)
cGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTCTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCg > 2:263764/1‑139 (MQ=255)
gAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCgg < 2:701701/139‑1 (MQ=255)
aTAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAg < 1:439636/139‑1 (MQ=255)
|
AAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAG > NC_000913/1322465‑1322730
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
