Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,325,247 |
(T)5→4 |
coding (502/1896 nt) |
yciQ → |
enhancer of membrane protein expression, putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,325,243 | 0 | T | . | 100.0%
| 115.6
/ NA
| 27 | coding (498/1896 nt) | yciQ | enhancer of membrane protein expression, putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (13/14); total (13/14) |
TGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTT > NC_000913/1325113‑1325342
|
tGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc < 1:203806/139‑1 (MQ=255)
aGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTtct > 1:73922/1‑139 (MQ=255)
tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg > 1:270333/1‑120 (MQ=255)
tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg < 2:270333/120‑1 (MQ=255)
aTTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCAttt > 2:205280/1‑139 (MQ=255)
tATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAatat > 2:703525/1‑121 (MQ=255)
tATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAatat < 1:703525/121‑1 (MQ=255)
aaaaaTGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGa > 1:220557/1‑139 (MQ=255)
gCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCtttt < 1:205280/139‑1 (MQ=255)
ttCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTAc < 1:426483/139‑1 (MQ=255)
tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg > 2:229256/1‑84 (MQ=255)
tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg < 1:229256/84‑1 (MQ=255)
gggggATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAgg > 2:797040/1‑139 (MQ=255)
ggATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCg < 1:797040/139‑1 (MQ=255)
aTTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac < 2:228278/139‑1 (MQ=255)
gATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACata < 2:220557/139‑1 (MQ=255)
aTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCa > 2:842055/1‑61 (MQ=255)
aTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCa < 1:842055/61‑1 (MQ=255)
aCGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac < 2:268624/118‑1 (MQ=255)
aCGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac > 1:268624/1‑118 (MQ=255)
tGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCtt > 2:339002/1‑139 (MQ=255)
cTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTa < 2:185815/85‑1 (MQ=255)
cTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTa > 1:185815/1‑85 (MQ=255)
ccGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg > 1:58174/1‑35 (MQ=255)
ccGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg < 2:58174/35‑1 (MQ=255)
ttGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACat > 2:283564/1‑98 (MQ=255)
ttGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACat < 1:283564/98‑1 (MQ=255)
|
TGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTT > NC_000913/1325113‑1325342
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
