Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,327,261 |
A→G |
E137G (GAA→GGA) |
rluB → |
23S rRNA pseudouridine(2605) synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,327,261 | 0 | A | G | 100.0%
| 171.3
/ NA
| 48 | E137G (GAA→GGA) | rluB | 23S rRNA pseudouridine(2605) synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24) |
TTTGACCGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAC > NC_000913/1327125‑1327394
|
tttGACCGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGAc > 1:26458/1‑139 (MQ=255)
ccGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGaa < 1:323966/139‑1 (MQ=255)
aaCTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg < 2:414100/139‑1 (MQ=255)
aaCTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg < 1:885287/139‑1 (MQ=255)
cgcACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGt < 1:797404/139‑1 (MQ=255)
cTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGtt < 2:488060/139‑1 (MQ=255)
aTTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTgacg > 1:701208/1‑139 (MQ=255)
gtgGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGt > 2:509035/1‑139 (MQ=255)
tgGTCTGCTGCTGTTCACCACCGACGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg < 2:264580/139‑1 (MQ=255)
tgGTCTGCCGCTGTTCACAAACGATTGTGAACTGGCAAACCGTTTAATGCTCCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg < 2:701208/139‑1 (MQ=255)
tgctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgc < 2:705133/81‑1 (MQ=255)
tgctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgc > 1:705133/1‑81 (MQ=255)
ctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTga > 2:19971/1‑98 (MQ=255)
ctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTga < 1:19971/98‑1 (MQ=255)
ttCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATg > 1:316765/1‑139 (MQ=255)
ccaccGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGaa < 1:672845/59‑1 (MQ=255)
ccaccGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGaa > 2:672845/1‑59 (MQ=255)
ccaccGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTcc > 1:861339/1‑139 (MQ=255)
aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg > 1:636271/1‑116 (MQ=255)
aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg < 2:636271/116‑1 (MQ=255)
aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGc > 2:56408/1‑139 (MQ=255)
aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGc > 1:711488/1‑139 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg < 1:348180/53‑1 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg > 2:348180/1‑53 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGt > 2:931116/1‑63 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGt < 1:931116/63‑1 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGAt < 2:10102/90‑1 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGAt > 1:10102/1‑90 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTc > 1:643628/1‑97 (MQ=255)
gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTc < 2:643628/97‑1 (MQ=255)
tttAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTc > 1:141500/1‑139 (MQ=255)
tttAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTc > 1:441527/1‑139 (MQ=255)
tAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg > 1:598127/1‑43 (MQ=255)
tAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg < 2:598127/43‑1 (MQ=255)
tGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcgg > 1:5042/1‑139 (MQ=255)
cACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcggcg < 1:509035/139‑1 (MQ=255)
ccAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcggcggcg > 1:312402/1‑139 (MQ=255)
ccAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcggcggcg > 2:202420/1‑139 (MQ=255)
cAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGa < 2:26458/139‑1 (MQ=255)
aGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAg > 1:316161/1‑139 (MQ=255)
ccGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAggg > 1:229024/1‑139 (MQ=255)
tGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCg < 1:530986/77‑1 (MQ=255)
tGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCg > 2:530986/1‑77 (MQ=255)
gAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCa < 1:169410/139‑1 (MQ=255)
aGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc < 2:229024/139‑1 (MQ=255)
aGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc < 1:202420/139‑1 (MQ=255)
aGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc < 2:141500/139‑1 (MQ=255)
aGTTGGACGTGAATATGCCGTGCGTGTATTTGGGCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc < 2:441527/139‑1 (MQ=255)
|
TTTGACCGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAC > NC_000913/1327125‑1327394
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
