Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 764 42.0 2018750 95.5% 1927906 109.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,325,247 (T)5→4 coding (502/1896 nt) yciQ → enhancer of membrane protein expression, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,325,2430T.100.0% 167.8 / NA 40coding (498/1896 nt)yciQenhancer of membrane protein expression, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/22);  total (18/22)

TTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGAAGAATTGAATCCCGCG  >  NC_000913/1325108‑1325367
                                                                                                                                       |                                                                                                                            
tttCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGtttt                                                                                                                           <  2:279605/139‑1 (MQ=255)
     tGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc                                                                                                                     <  1:898178/139‑1 (MQ=255)
     tGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc                                                                                                                     <  2:439852/139‑1 (MQ=255)
        cTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg                                                                                                                  <  2:246057/139‑1 (MQ=255)
        cTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg                                                                                                                  <  2:153159/139‑1 (MQ=255)
         tCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGt                                                                                                                 >  1:293978/1‑139 (MQ=255)
                     tCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTg                                                                                                     <  2:487437/139‑1 (MQ=255)
                     tCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTg                                                                                                     <  2:930217/139‑1 (MQ=255)
                       aTTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg                                                                                                                  >  1:911347/1‑124 (MQ=255)
                       aTTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAg                                                                                                                  <  2:911347/124‑1 (MQ=255)
                           tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGt                                                                                                                              >  2:559820/1‑109 (MQ=255)
                           tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGt                                                                                                                              <  1:559820/109‑1 (MQ=255)
                                   aTTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc                                                                                                                     >  2:674465/1‑109 (MQ=255)
                                   aTTATCAGGTTAAAAATGCTTGCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc                                                                                                                     <  1:674465/109‑1 (MQ=255)
                                      aTCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGc                                                                                    <  2:422362/139‑1 (MQ=255)
                                          ggTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGaaa                                                                                >  1:813053/1‑139 (MQ=255)
                                           gTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAAt                                                                               >  1:923312/1‑139 (MQ=255)
                                                           tGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGga                                                               <  2:813053/139‑1 (MQ=255)
                                                            gCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCg                                                                             <  2:185210/124‑1 (MQ=255)
                                                            gCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCg                                                                             >  1:185210/1‑124 (MQ=255)
                                                               tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc                                                                                                                     <  2:514426/81‑1 (MQ=255)
                                                               tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTtctctc                                                                                                                     >  1:514426/1‑81 (MQ=255)
                                                                 aGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaa                                                            <  2:20704/136‑1 (MQ=255)
                                                                 aGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaa                                                            >  1:20704/1‑136 (MQ=255)
                                                                    gggATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGc                                                      <  1:158013/139‑1 (MQ=255)
                                                                       aTTCTGATCTGCTAGTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                                                   <  1:327519/139‑1 (MQ=255)
                                                                       aTTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACGCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                                                   >  1:652946/1‑139 (MQ=255)
                                                                       aTTATGATCTGCTAATCTGGAACGTTACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                                                   <  2:652946/139‑1 (MQ=255)
                                                                        ttCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGaca                                                  <  2:923312/139‑1 (MQ=255)
                                                                                  cTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTctct                                                                                                            <  2:49354/71‑1 (MQ=255)
                                                                                  cTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTctct                                                                                                            >  1:49354/1‑71 (MQ=255)
                                                                                  cTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTaa                                        >  2:754002/1‑139 (MQ=255)
                                                                                     aTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATgg                                     >  1:315212/1‑139 (MQ=255)
                                                                                          gAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCa                                >  2:956027/1‑139 (MQ=255)
                                                                                               tGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaaga                                                          >  1:820735/1‑108 (MQ=255)
                                                                                               tGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaaga                                                          <  2:820735/108‑1 (MQ=255)
                                                                                                 aCCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGa                                                    <  2:925546/112‑1 (MQ=255)
                                                                                                 aCCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGa                                                    >  1:925546/1‑112 (MQ=255)
                                                                                                 aCCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGa                         >  2:914234/1‑139 (MQ=255)
                                                                                                      tAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAgg                                                        >  1:332085/1‑103 (MQ=255)
                                                                                                      tAACCACTGGCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAgg                                                        <  2:332085/103‑1 (MQ=255)
                                                                                                               gCCGTTTGAAATTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTACTATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGACGAATTg           >  1:462080/1‑139 (MQ=255)
                                                                                                                        aaTTTATAAGACCCG‑TTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGAAGAATTGAATCCcgcg  <  2:315212/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
TTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGAAGAATTGAATCCCGCG  >  NC_000913/1325108‑1325367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A