Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,317,027 C→T *950* (TAG→TAA)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,317,0270CT100.0% 167.9 / NA 52*950* (TAG→TAA) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

CCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAA  >  NC_000913/2316902‑2317158
                                                                                                                             |                                                                                                                                   
ccTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                        <  1:460425/139‑1 (MQ=255)
           cTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAcc                                                                                                             >  2:900074/1‑139 (MQ=255)
                   cccATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGc                                                                                                     >  2:540813/1‑139 (MQ=255)
                    ccATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                        >  2:130490/1‑119 (MQ=255)
                    ccATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                        <  1:130490/119‑1 (MQ=255)
                    ccATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCa                                                                                                    <  2:348194/139‑1 (MQ=255)
                        tattTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCAtata                                                                                                >  1:103882/1‑139 (MQ=255)
                           ttCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACg                                                                                             >  2:609358/1‑139 (MQ=255)
                              aacagATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATc                                                                                                                             <  2:1027215/104‑1 (MQ=255)
                              aacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTAGGAATc                                                                                                                             >  1:1027215/1‑104 (MQ=255)
                                caaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGc                                                                                        <  1:540813/139‑1 (MQ=255)
                                  aaTTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGt                                                                                      <  1:1003345/139‑1 (MQ=255)
                                             aGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCct                                                                                                           <  2:1273164/107‑1 (MQ=255)
                                             aGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCct                                                                                                           >  1:1273164/1‑107 (MQ=255)
                                             aGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                        <  1:833904/110‑1 (MQ=255)
                                             aGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                        >  2:833904/1‑110 (MQ=255)
                                               tAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATcaca                                                                         >  1:1404161/1‑139 (MQ=255)
                                                 ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATc                                                                       >  1:701702/1‑139 (MQ=255)
                                                  gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                        <  2:159737/89‑1 (MQ=255)
                                                  gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                        >  1:159737/1‑89 (MQ=255)
                                                  gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCata                                                                                                  <  2:362129/111‑1 (MQ=255)
                                                  gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCata                                                                                                  >  1:362129/1‑111 (MQ=255)
                                                  gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc                                                                      >  2:253898/1‑139 (MQ=255)
                                                       aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGt                                                                                      >  2:340725/1‑118 (MQ=255)
                                                       aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGt                                                                                      <  1:340725/118‑1 (MQ=255)
                                                       aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc                                                                      <  2:245835/134‑1 (MQ=255)
                                                       aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc                                                                      >  1:245835/1‑134 (MQ=255)
                                                       aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGt                                                                 >  1:1534923/1‑139 (MQ=255)
                                                            aCGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                            >  2:778057/1‑91 (MQ=255)
                                                            aCGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                            <  1:778057/91‑1 (MQ=255)
                                                             cgcgTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCCCGGCATATAACGTCAGCGTCTGTTTTATTACATCCAGCCTTACCgg                                                           >  2:1528409/1‑139 (MQ=255)
                                                                gTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                        <  2:727070/91‑1 (MQ=255)
                                                                gTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                        >  1:727070/1‑91 (MQ=255)
                                                                 tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATcaca                                                                         >  1:370783/1‑121 (MQ=255)
                                                                 tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATcaca                                                                         <  2:370783/121‑1 (MQ=255)
                                                                 tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTc                                                       >  2:597778/1‑139 (MQ=255)
                                                                          cATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAg                                              <  2:701702/139‑1 (MQ=255)
                                                                                     tGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATAcc                                   <  1:390274/139‑1 (MQ=255)
                                                                                           gAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCgg                                                                                                      >  2:1199924/1‑66 (MQ=255)
                                                                                           gAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCgg                                                                                                      <  1:1199924/66‑1 (MQ=255)
                                                                                            aaTGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTcc                            >  2:1084023/1‑139 (MQ=255)
                                                                                            aaTGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTcc                            >  2:1220601/1‑139 (MQ=255)
                                                                                               gCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGa                         <  1:1084023/139‑1 (MQ=255)
                                                                                                    aTGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCg                    <  1:609358/139‑1 (MQ=255)
                                                                                                      gcggcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc                                                                                     >  1:460583/1‑72 (MQ=255)
                                                                                                      gcggcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc                                                                                     <  2:460583/72‑1 (MQ=255)
                                                                                                              aaCGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTctct          >  2:1317547/1‑139 (MQ=255)
                                                                                                                   cTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGc     >  1:119595/1‑139 (MQ=255)
                                                                                                                    ttATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGcc    <  1:900074/139‑1 (MQ=255)
                                                                                                                      aTCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCaa  >  2:1065260/1‑139 (MQ=255)
                                                                                                                      aTCCGACTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAAc                                                                                              <  1:994898/47‑1 (MQ=255)
                                                                                                                      aTCCGACTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAAc                                                                                              >  2:994898/1‑47 (MQ=255)
                                                                                                                             |                                                                                                                                   
CCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAA  >  NC_000913/2316902‑2317158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A