Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,317,027 |
C→T |
*950* (TAG→TAA) |
rcsC ← |
hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,317,027 | 0 | C | T | 100.0%
| 127.3
/ NA
| 40 | *950* (TAG→TAA) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/19); total (21/19) |
CTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCC > NC_000913/2316913‑2317156
|
cTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAcc > 1:844049/1‑139 (MQ=255)
gATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctct < 1:1072829/139‑1 (MQ=255)
aTTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc > 2:52079/1‑139 (MQ=255)
ggCCAGATAAGACGCGTCAGCGTCGCCTCTGGCATTTGCACTGAATGCCGGAAGCAGCGTAAACGCCTTATTCGTATTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAAAGTCAGCGTCCGTTTTACCACATc > 2:1302149/1‑139 (MQ=37)
ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATc < 1:981884/85‑1 (MQ=255)
ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATc > 2:981884/1‑85 (MQ=255)
ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCgg < 2:89137/108‑1 (MQ=255)
ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCgg > 1:89137/1‑108 (MQ=255)
gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTc < 1:70699/118‑1 (MQ=255)
gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTc > 2:70699/1‑118 (MQ=255)
gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc > 2:1194167/1‑139 (MQ=255)
ccAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCa < 1:52079/139‑1 (MQ=255)
ccAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCa < 1:1194167/139‑1 (MQ=255)
aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc < 2:722588/100‑1 (MQ=255)
aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc > 1:722588/1‑100 (MQ=255)
gACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAc < 2:747138/90‑1 (MQ=255)
gACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAc > 1:747138/1‑90 (MQ=255)
aCGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCg > 2:856194/1‑139 (MQ=255)
cgTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGa > 2:57044/1‑77 (MQ=255)
cgTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGa < 1:57044/77‑1 (MQ=255)
tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctc < 1:1321647/88‑1 (MQ=255)
tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctc > 2:1321647/1‑88 (MQ=255)
cGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCATGACCCTCTCGGCATATAACGTCAGCATCTGTTTTATCACATCCAGCGTTACCGGCTTCGACa < 1:856194/139‑1 (MQ=255)
tttGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctc < 2:698797/70‑1 (MQ=255)
tttGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctc > 1:698797/1‑70 (MQ=255)
gCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCg < 1:1319614/139‑1 (MQ=255)
aaTGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAgg < 1:1335177/118‑1 (MQ=255)
aaTGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAgg > 2:1335177/1‑118 (MQ=255)
aaTGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTcc > 1:1027384/1‑139 (MQ=255)
gCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGa > 2:1275905/1‑139 (MQ=255)
cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc > 1:7698/1‑77 (MQ=255)
cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc < 2:7698/77‑1 (MQ=255)
aTGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc > 2:656877/1‑55 (MQ=255)
aTGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc < 1:656877/55‑1 (MQ=255)
gcggcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc < 1:825166/72‑1 (MQ=255)
gcggcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTc > 2:825166/1‑72 (MQ=255)
gcggcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCgct > 1:427917/1‑139 (MQ=255)
gcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATc > 2:1052519/1‑83 (MQ=255)
gcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATc < 1:1052519/83‑1 (MQ=255)
ttATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGcc < 2:427917/139‑1 (MQ=255)
|
CTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCC > NC_000913/2316913‑2317156
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
