Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,027	C→T	950 (TAG→TAA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,027	0	C	T	100.0%	110.2 / NA	35	950 (TAG→TAA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/15); total (20/15)

GATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACG  >  NC_000913/2316917‑2317160

gATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCctct                                                                                                           <  1:285362/139‑1 (MQ=255)

  ttCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCg                                                                                                         >  1:1063826/1‑139 (MQ=255)

              caacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                          <  2:566547/126‑1 (MQ=255)

              caacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                          >  2:330307/1‑126 (MQ=255)

              caacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                          <  1:330307/126‑1 (MQ=255)

              caacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCtctc                                                                                                          >  1:566547/1‑126 (MQ=255)

              caacaaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTc                                                                                             <  1:396311/139‑1 (MQ=255)

                 caaATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGc                                                                                          >  2:1037031/1‑139 (MQ=255)

                              aGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATca                                                                             >  2:994462/1‑139 (MQ=255)

                                  ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTTAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATc                                                                         >  1:726009/1‑139 (MQ=255)

                                   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                          >  1:701071/1‑89 (MQ=255)

                                   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCcgcg                                                                                                                          <  2:701071/89‑1 (MQ=255)

                                   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc                                                                        >  1:1001372/1‑139 (MQ=255)

                                   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc                                                                        >  2:874594/1‑139 (MQ=255)

                                   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATcc                                                                        >  1:910607/1‑139 (MQ=255)

                                        aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              <  1:317738/96‑1 (MQ=255)

                                        aTAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGAccc                                                                                                              >  2:317738/1‑96 (MQ=255)

                                            gACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTAcc                                                               <  2:1001372/139‑1 (MQ=255)

                                                  tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCa                                                                       >  2:154794/1‑125 (MQ=255)

                                                  tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCa                                                                       <  1:154794/125‑1 (MQ=255)

                                                  tCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTc                                                         >  1:964683/1‑139 (MQ=255)

                                                   cAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCata                                                                                                    <  2:610391/95‑1 (MQ=255)

                                                   cAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCata                                                                                                    >  1:610391/1‑95 (MQ=255)

                                                     gCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGAc                                                      <  1:874594/139‑1 (MQ=255)

                                                                  cATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACAt                                                                          >  1:552315/1‑106 (MQ=255)

                                                                  cATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACAt                                                                          <  2:552315/106‑1 (MQ=255)

                                                                            gAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGa                                                                                                                         <  2:122309/49‑1 (MQ=255)

                                                                            gAATGCCGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGa                                                                                                                         >  1:122309/1‑49 (MQ=255)

                                                                                  cGGATGCGGCGTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGaca                         >  1:825464/1‑139 (MQ=255)

                                                                                       gcggcgTAAACGCCTTATCCGTCTTACGAATACCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCgct                    >  2:601161/1‑139 (MQ=255)

                                                                                         ggcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCgctgc                  >  2:111777/1‑139 (MQ=255)

                                                                                          gcgTAAACGCCTTATCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCgctgct                 <  2:825464/139‑1 (MQ=255)

                                                                                                       aTCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCaa    <  2:1063826/139‑1 (MQ=255)

                                                                                                       aTCCGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCaa    >  2:486725/1‑139 (MQ=255)

                                                                                                         ccGTCTTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACg  <  2:964683/139‑1 (MQ=255)

GATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACG  >  NC_000913/2316917‑2317160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A