Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,400,773 |
A→G |
R105R (CGT→CGC) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,400,773 | 0 | A | G | 100.0%
| 144.3
/ NA
| 42 | R105R (CGT→CGC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/21); total (21/21) |
ATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTA > NC_000913/3400642‑3400893
|
aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa > 2:1528576/1‑139 (MQ=255)
aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa > 1:1416807/1‑139 (MQ=255)
aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa < 1:454743/139‑1 (MQ=255)
gCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt > 1:223143/1‑139 (MQ=255)
aGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTg < 2:718571/139‑1 (MQ=255)
gCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGc > 2:862795/1‑139 (MQ=255)
tCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtgtg < 1:409881/139‑1 (MQ=255)
ttcAATCAGGAAGGCTTCACGGGCACCAGCTCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGt > 2:1256928/1‑139 (MQ=255)
aTCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtga > 2:153539/1‑139 (MQ=255)
aTCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtga < 1:1528576/139‑1 (MQ=255)
cAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 2:840582/1‑139 (MQ=255)
gAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt < 2:1428169/108‑1 (MQ=255)
gAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt > 1:1428169/1‑108 (MQ=255)
ggcTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGc < 1:1133422/137‑1 (MQ=255)
ggcTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGc > 2:1133422/3‑139 (MQ=255)
ttCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg > 1:1478441/1‑139 (MQ=255)
cACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt > 1:784912/1‑108 (MQ=255)
cACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt < 2:784912/108‑1 (MQ=255)
cACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTg < 1:177735/101‑1 (MQ=255)
cACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTg > 2:177735/1‑101 (MQ=255)
cACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttt > 2:6596/1‑139 (MQ=255)
gcgcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg < 1:862795/139‑1 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCAc > 2:1369212/1‑103 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCAc < 1:1369212/103‑1 (MQ=255)
cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACgaag > 2:186996/1‑139 (MQ=255)
cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGACACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt < 1:114910/132‑1 (MQ=255)
cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGACACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt > 2:114910/1‑132 (MQ=255)
tGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg < 1:153539/139‑1 (MQ=255)
ggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGa < 1:6596/139‑1 (MQ=255)
gTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg < 1:1306637/120‑1 (MQ=255)
gTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg > 2:1306637/1‑120 (MQ=255)
gcgcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtg < 1:1288463/57‑1 (MQ=255)
gcgcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtg > 2:1288463/1‑57 (MQ=255)
gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg < 1:927260/115‑1 (MQ=255)
gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg > 2:927260/1‑115 (MQ=255)
gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgca < 2:223143/139‑1 (MQ=255)
ccAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag < 2:1478441/139‑1 (MQ=255)
aCCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc < 2:1141961/113‑1 (MQ=255)
aCCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc > 1:1141961/1‑113 (MQ=255)
cAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTa < 2:362015/139‑1 (MQ=255)
agcgcgcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCa < 2:1001213/36‑1 (MQ=255)
agcgcgcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCa > 1:1001213/3‑38 (MQ=255)
|
ATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTA > NC_000913/3400642‑3400893
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
