Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,773	A→G	R105R (CGT→CGC)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,773	0	A	G	100.0%	127.6 / NA	36	R105R (CGT→CGC)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/19); total (17/19)

ATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCC  >  NC_000913/3400642‑3400899

aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                                                                                                                         <  2:371979/139‑1 (MQ=255)

aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                                                                                                                         <  2:315936/139‑1 (MQ=255)

  tGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAg                                                                                                                       <  1:77117/139‑1 (MQ=255)

    cGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCt                                                                                                                     >  1:1082382/1‑139 (MQ=255)

           cATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGAGCATAAAGCTGTTGCtg                                                                                                              <  2:636610/139‑1 (MQ=255)

                gTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCAc                                                                                                         <  1:884830/139‑1 (MQ=255)

                          cAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                                                                                               <  2:1082382/139‑1 (MQ=255)

                           aGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatga                                                                                              >  1:964292/1‑139 (MQ=255)

                               aGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg                                                                                          <  2:964292/139‑1 (MQ=255)

                                 aCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCt                                                                                        >  2:24046/1‑139 (MQ=255)

                                             ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttttt                                                                            <  2:89764/139‑1 (MQ=255)

                                                      tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCt                                                                                        <  2:368974/118‑1 (MQ=255)

                                                      tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCt                                                                                        >  1:368974/1‑118 (MQ=255)

                                                      tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg                                                                         >  1:296781/1‑133 (MQ=255)

                                                      tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg                                                                         <  2:296781/133‑1 (MQ=255)

                                                        cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt                                                                        <  2:349106/132‑1 (MQ=255)

                                                        cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt                                                                        >  1:349106/1‑132 (MQ=255)

                                                        cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACgaag                                                                 >  2:831297/1‑139 (MQ=255)

                                                         gcgGATTCACGAATTGCGCGGCGTTCAACCTGGG‑CGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACgaagaa                                                               <  2:1063202/139‑1 (MQ=255)

                                                                      ttGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAAc                                                   >  2:761590/1‑139 (MQ=255)

                                                                       tGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg                                                  >  1:1084616/1‑139 (MQ=255)

                                                                           cggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGt                                              >  1:535843/1‑139 (MQ=255)

                                                                                      cTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATaa                                                    <  1:468101/122‑1 (MQ=255)

                                                                                      cTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATaa                                                    >  2:468101/1‑122 (MQ=255)

                                                                                        gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCa                                        >  2:600622/1‑132 (MQ=255)

                                                                                        gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCa                                        <  1:600622/132‑1 (MQ=255)

                                                                                               gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgca                          >  2:225223/1‑139 (MQ=255)

                                                                                               gcCAACCGGCACACAAACCAGAAAGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGTCGAATGgca                          >  1:991629/1‑139 (MQ=255)

                                                                                                ccAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag                         <  2:535843/139‑1 (MQ=255)

                                                                                                                  aGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTAc       <  1:761590/139‑1 (MQ=255)

                                                                                                                      cgcgcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGcc   <  1:866907/139‑1 (MQ=255)

                                                                                                                       gcgcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGccc  >  1:281041/1‑139 (MQ=255)

                                                                                                                         gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAAc                                                   >  2:277957/1‑88 (MQ=255)

                                                                                                                         gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAAc                                                   <  1:277957/88‑1 (MQ=255)

                                                                                                                         gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCg          <  2:508615/129‑1 (MQ=255)

                                                                                                                         gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCg          >  1:508615/1‑129 (MQ=255)

ATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCC  >  NC_000913/3400642‑3400899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A