Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,400,773 |
A→G |
R105R (CGT→CGC) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,400,773 | 0 | A | G | 100.0%
| 133.4
/ NA
| 38 | R105R (CGT→CGC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/19); total (19/19) |
CGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCC > NC_000913/3400646‑3400899
|
cGGCAGCCATCGGTTCTTCATTCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCt > 2:472633/1‑139 (MQ=255)
ggCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTg < 1:682423/139‑1 (MQ=255)
tcAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGtt < 2:308024/139‑1 (MQ=255)
gAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGATtgatgacg > 2:671470/1‑137 (MQ=255)
aaGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtgt < 1:1022527/121‑1 (MQ=255)
aaGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtgt > 2:1022527/1‑121 (MQ=255)
gggCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt > 2:263083/1‑139 (MQ=255)
cgcCCTGCGCGGACTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCAAACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt < 1:472633/139‑1 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACtt < 1:759066/105‑1 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACtt > 2:759066/1‑105 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAc > 1:508755/1‑139 (MQ=255)
tGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGtt < 2:755811/89‑1 (MQ=255)
tGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGtt > 1:755811/1‑89 (MQ=255)
cgcgGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACtt > 1:565475/1‑84 (MQ=255)
cgcgGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACtt < 2:565475/84‑1 (MQ=255)
cTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTggg < 1:261698/139‑1 (MQ=255)
gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc > 2:690800/1‑124 (MQ=255)
gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc < 1:690800/124‑1 (MQ=255)
gTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg < 1:336450/75‑1 (MQ=255)
gTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 2:336450/1‑75 (MQ=255)
cgcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgc > 1:340326/1‑139 (MQ=255)
cgcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgc > 1:578721/1‑139 (MQ=255)
gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg < 2:993847/70‑1 (MQ=255)
gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 1:993847/1‑70 (MQ=255)
ccAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag < 2:340326/139‑1 (MQ=255)
gCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgca > 2:625597/1‑131 (MQ=255)
gCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgca < 1:625597/131‑1 (MQ=255)
aCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtgtg > 2:399454/1‑41 (MQ=255)
aCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtgtg < 1:399454/41‑1 (MQ=255)
aGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTAc < 2:508755/139‑1 (MQ=255)
cgcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg > 1:727099/1‑90 (MQ=255)
cgcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg < 2:727099/90‑1 (MQ=255)
gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTcggt < 2:1029453/67‑4 (MQ=255)
gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTcggt > 1:1029453/1‑64 (MQ=255)
gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGccc < 2:279079/137‑1 (MQ=255)
gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGccc > 1:279079/1‑137 (MQ=255)
gggCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg > 1:840270/1‑87 (MQ=255)
gggCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg < 2:840270/87‑1 (MQ=255)
|
CGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCC > NC_000913/3400646‑3400899
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
