Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,690	C→T	256 (TAG→TAA)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,690	0	C	T	100.0%	187.5 / NA	58	256 (TAG→TAA)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/31); total (27/31)

AAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGTTT  >  NC_000913/4510558‑4510822

aaaGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTc                                                                                                                                <  2:942512/139‑1 (MQ=255)

       gcGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGc                                                                                                                         >  1:199920/1‑139 (MQ=255)

               aCGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  1:664870/139‑1 (MQ=255)

               aCGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  1:665630/139‑1 (MQ=255)

                         ttGGTAATCTTTCAGAGTCCGGAATAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                       <  1:858067/139‑1 (MQ=255)

                               aTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 <  1:886350/139‑1 (MQ=255)

                                 cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGCACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  2:327526/121‑1 (MQ=255)

                                 cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 >  1:327526/1‑121 (MQ=255)

                                 cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               >  2:14084/1‑139 (MQ=255)

                                 cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               >  2:1332166/1‑139 (MQ=255)

                                   ttCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCggg                                                                                             <  2:199920/139‑1 (MQ=255)

                                      agagTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                                <  2:132956/117‑1 (MQ=255)

                                      agagTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                                >  1:132956/1‑117 (MQ=255)

                                      agagTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGgtg                                                                                          >  2:1289381/1‑139 (MQ=255)

                                          tCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTAtt                                                                                      >  2:828769/1‑139 (MQ=255)

                                            cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGgtgt                                                                                         <  1:966712/134‑1 (MQ=255)

                                            cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGgtgt                                                                                         >  2:966712/1‑134 (MQ=255)

                                             ggAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTcc                                                                                   >  1:154308/1‑139 (MQ=255)

                                                    ttCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCa                                                                            >  2:1511770/1‑139 (MQ=255)

                                                      cAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACg                                                                          <  1:14084/139‑1 (MQ=255)

                                                        gCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCgggg                                                                                            <  2:718646/119‑1 (MQ=255)

                                                        gCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCgggg                                                                                            >  1:718646/1‑119 (MQ=255)

                                                                       ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  1:1332166/139‑1 (MQ=255)

                                                                       ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:154308/139‑1 (MQ=255)

                                                                        gCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGc                                                                                                  >  1:516998/1‑97 (MQ=255)

                                                                        gCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGc                                                                                                  <  2:516998/97‑1 (MQ=255)

                                                                         cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:450837/137‑1 (MQ=255)

                                                                         cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         >  1:450837/1‑137 (MQ=255)

                                                                         cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCa                                                       >  1:606166/1‑139 (MQ=255)

                                                                         cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCa                                                       >  1:608510/1‑139 (MQ=255)

                                                                             cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  1:291112/77‑1 (MQ=255)

                                                                             cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 >  2:291112/1‑77 (MQ=255)

                                                                             cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  1:1286860/133‑1 (MQ=255)

                                                                             cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         >  2:1286860/1‑133 (MQ=255)

                                                                             cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:769435/133‑1 (MQ=255)

                                                                             cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         >  1:769435/1‑133 (MQ=255)

                                                                                         cagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCAc                                       <  1:828769/139‑1 (MQ=255)

                                                                                              tcattcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTaac                                                                                                                                  <  2:920514/43‑3 (MQ=255)

                                                                                              tcattcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTAcc                                                                                                                                  >  1:920514/1‑43 (MQ=255)

                                                                                                 ttcaGTCGTGGTTTGGTTCTTACGGCCTGTACAATTTACCTCATTAGGCACATCGGCCTGcc                                                                                                            <  1:670803/62‑1 (MQ=255)

                                                                                                 ttcaGTCGTGGTTTGGTTCTTACGGCCTGTACAATTTACCTCATTAGGCACATCGGCCTGcc                                                                                                            >  2:670803/1‑62 (MQ=255)

                                                                                                      tCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCAc                                       >  1:778291/1‑126 (MQ=255)

                                                                                                      tCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCAc                                       <  2:778291/126‑1 (MQ=255)

                                                                                                        gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 >  1:777751/1‑66 (MQ=255)

                                                                                                        gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 <  2:777751/66‑1 (MQ=255)

                                                                                                        gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGcc                        <  1:1149545/139‑1 (MQ=255)

                                                                                                        gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGcc                        <  2:608510/139‑1 (MQ=255)

                                                                                                        gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGcc                        <  1:1511770/139‑1 (MQ=255)

                                                                                                         tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 >  2:153907/1‑65 (MQ=255)

                                                                                                         tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 <  1:153907/65‑1 (MQ=255)

                                                                                                         tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 >  2:390577/1‑65 (MQ=255)

                                                                                                         tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 <  1:390577/65‑1 (MQ=255)

                                                                                                           gtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               <  2:1344880/65‑1 (MQ=255)

                                                                                                           gtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               >  1:1344880/1‑65 (MQ=255)

                                                                                                              tggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTgcgc                  <  1:668486/139‑1 (MQ=255)

                                                                                                                              tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTg                                                 <  1:738234/92‑1 (MQ=255)

                                                                                                                              tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTg                                                 >  2:738234/1‑92 (MQ=255)

                                                                                                                              tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGttt  >  1:1023167/1‑139 (MQ=255)

AAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGTTT  >  NC_000913/4510558‑4510822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A