Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,146	C→T	W104* (TGG→TGA)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,146	0	C	T	100.0%	99.5 / NA	32	W104* (TGG→TGA)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/17); total (15/17)

AAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAA  >  NC_000913/4511014‑4511280

aaaTGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGa                                                                                                                                  >  1:721542/1‑139 (MQ=255)

    gcgcgcTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTAc                                                                                                                              >  2:623796/1‑139 (MQ=255)

     cgcgcTAGCGCTGACCGCCGGAAAGCTCAGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACg                                                                                                                             <  2:480639/139‑1 (MQ=255)

       cgctggcgctgACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGAc                                                                                                                           <  2:266382/139‑1 (MQ=255)

                  aCCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                                <  1:389094/139‑1 (MQ=255)

                          aaaGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTg                                                                                                        <  1:29892/139‑1 (MQ=255)

                            aGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                                      <  1:332030/139‑1 (MQ=255)

                              cTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTg                                                                                                        >  2:461679/1‑135 (MQ=255)

                              cTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTg                                                                                                        <  1:461679/135‑1 (MQ=255)

                                         gacgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                                         <  2:346848/139‑1 (MQ=255)

                                           agaACGGCAAGATGATTGATCCGGGTCTGGGTCATGGCGACATTAGCTCATGCATTGGCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                                       <  1:771480/138‑1 (MQ=255)

                                                    aGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAAt                                                                              <  1:149909/139‑1 (MQ=255)

                                                      atgatTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATgg                                                                            <  1:433530/139‑1 (MQ=255)

                                                                   gTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcagca                                                               >  2:325690/1‑139 (MQ=255)

                                                                       ggTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGc                                                                         >  1:422350/1‑125 (MQ=255)

                                                                       ggTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGc                                                                         <  2:422350/125‑1 (MQ=255)

                                                                       ggTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGGTAAATGGTGCTGAGGCAGCAGCGa                                                           >  1:344206/1‑139 (MQ=255)

                                                                        gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                                          >  1:402070/1‑139 (MQ=255)

                                                                        gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                                          >  2:366267/1‑139 (MQ=255)

                                                                                              gCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGcgcg                                    >  2:120162/1‑139 (MQ=255)

                                                                                              gCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGcgcg                                    >  1:281366/1‑139 (MQ=255)

                                                                                                  tGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                <  2:692/139‑1 (MQ=255)

                                                                                                      ttCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            <  1:623796/139‑1 (MQ=255)

                                                                                                      ttCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            <  1:366267/139‑1 (MQ=255)

                                                                                                       tCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa                           <  1:325690/139‑1 (MQ=255)

                                                                                                        cAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGAATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCat                          >  2:120956/1‑139 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt                    >  2:45551/1‑139 (MQ=255)

                                                                                                                     cccccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTa             >  1:234332/1‑139 (MQ=255)

                                                                                                                          agagTGACAGTCAGGGATTACGACCATACGAAACCAGCCCCTGGACTGTGATCCCCTCTGCCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCAAACTGGCGCGATGAGAGCATATTTATGGGATTATCGcc        <  1:603956/139‑1 (MQ=255)

                                                                                                                                aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                               >  2:290081/1‑46 (MQ=255)

                                                                                                                                aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                               <  1:290081/46‑1 (MQ=255)

                                                                                                                                aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGaaa  >  1:701306/1‑139 (MQ=255)

AAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAA  >  NC_000913/4511014‑4511280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A