Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,146	C→T	W104* (TGG→TGA)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,146	0	C	T	100.0%	98.9 / NA	32	W104* (TGG→TGA)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/18); total (14/18)

AAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTA  >  NC_000913/4511014‑4511269

aaaTGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGa                                                                                                                       >  1:516827/1‑139 (MQ=255)

 aaTGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGAt                                                                                                                      <  2:552310/139‑1 (MQ=255)

       cgctggcgctgACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGAc                                                                                                                <  1:301513/139‑1 (MQ=255)

                      ccgGAAAGCTCGGTTAACCGACGAACGTCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                     >  1:603547/1‑135 (MQ=255)

                      ccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCt                                                                                                 >  1:772000/1‑139 (MQ=255)

                      ccgGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCAGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                     <  2:603547/135‑1 (MQ=255)

                            aGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                           <  2:179774/139‑1 (MQ=255)

                                     aaCCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATcc                                                                                  <  2:516827/139‑1 (MQ=255)

                                         gacgaACGGCAAGATGATTGATCCGTGTCTGGTTCATGGCGACATTAGCTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATGCCctc                                                                              <  1:312993/139‑1 (MQ=255)

                                           cgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                            <  2:594648/139‑1 (MQ=255)

                                           cgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                            <  1:939131/139‑1 (MQ=255)

                                             aaCGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGc                                                                          >  2:198077/1‑139 (MQ=255)

                                                   aaGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTTGACTGTGATCCCCTCTGGCGTTaaa                                                                    <  2:181781/139‑1 (MQ=255)

                                                   aaGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTaaa                                                                    <  2:772000/139‑1 (MQ=255)

                                                                                 gACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCgg                                      >  2:984950/1‑139 (MQ=255)

                                                                                   cATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                     >  2:583697/1‑74 (MQ=255)

                                                                                   cATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                     <  1:583697/74‑1 (MQ=255)

                                                                                             tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGATAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGgcgc                          >  2:57513/1‑139 (MQ=255)

                                                                                                  tGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                     <  1:984950/139‑1 (MQ=255)

                                                                                                    tCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATgaga                   >  1:474493/1‑139 (MQ=255)

                                                                                                    tCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATgaga                   >  2:884640/1‑139 (MQ=255)

                                                                                                         aGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATAc                                                                                                           <  1:879448/46‑1 (MQ=255)

                                                                                                         aGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATAc                                                                                                           >  2:879448/1‑46 (MQ=255)

                                                                                                            ggAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATAttt           <  2:474493/139‑1 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGATAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt         <  1:57513/139‑1 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                    <  1:124587/64‑1 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                    >  2:124587/1‑64 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                 >  2:478699/1‑131 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                 >  1:763090/1‑131 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                 <  1:478699/131‑1 (MQ=255)

                                                                                                              agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                 <  2:763090/131‑1 (MQ=255)

                                                                                                                     cccccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTa  >  2:63179/1‑139 (MQ=255)

AAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTA  >  NC_000913/4511014‑4511269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A