Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,511,146 C→T W104* (TGG→TGA)  fecE ← ferric citrate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,511,1460CT100.0% 205.8 / NA 64W104* (TGG→TGA) fecEferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/32);  total (32/32)

ACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAA  >  NC_000913/4511032‑4511280
                                                                                                                  |                                                                                                                                      
aCCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                                <  2:493577/139‑1 (MQ=255)
          aGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                                      >  2:500233/1‑139 (MQ=255)
                 ttAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                               <  2:759162/139‑1 (MQ=255)
                      cgacgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          <  1:104685/139‑1 (MQ=255)
                       gacgaACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                                         <  2:67072/139‑1 (MQ=255)
                                 aaGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTaaa                                                                               <  1:500233/139‑1 (MQ=255)
                                          gatCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                                       <  1:1021641/122‑1 (MQ=255)
                                          gatCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTg                                                                                       >  2:1021641/1‑122 (MQ=255)
                                             ccGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCt                                                                                                         <  2:642544/101‑1 (MQ=255)
                                             ccGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCt                                                                                                         >  1:642544/1‑101 (MQ=255)
                                              cGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGa                                                                                                                            >  1:705605/1‑81 (MQ=255)
                                              cGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGa                                                                                                                            <  2:705605/81‑1 (MQ=255)
                                              cGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgca                                                                  >  1:719164/1‑139 (MQ=255)
                                               gggTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                                 >  1:771543/1‑139 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                                         >  1:1200699/1‑108 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                                         <  2:1200699/108‑1 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          >  1:1059974/1‑107 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          <  2:1059974/107‑1 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                                 >  2:1265511/1‑132 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                                 <  1:1265511/132‑1 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                                          >  1:1279350/1‑139 (MQ=255)
                                                      gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                                          >  1:64881/1‑139 (MQ=255)
                                                                    tAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                                <  2:689965/71‑1 (MQ=255)
                                                                    tAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                                >  1:689965/1‑71 (MQ=255)
                                                                    tAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCaa                                            <  1:1244607/139‑1 (MQ=255)
                                                                           tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                                         >  2:121398/1‑87 (MQ=255)
                                                                           tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCctc                                                                                         <  1:121398/87‑1 (MQ=255)
                                                                           tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGgcgc                                     >  2:651558/1‑139 (MQ=255)
                                                                           tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGgcgc                                     >  1:27316/1‑139 (MQ=255)
                                                                            gCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCAt                                                                                                                        >  2:1196606/1‑55 (MQ=255)
                                                                            gCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCAt                                                                                                                        <  1:1196606/55‑1 (MQ=255)
                                                                             cATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACg                                                                                                                             <  2:1343997/49‑1 (MQ=255)
                                                                             cATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACg                                                                                                                             >  1:1343997/1‑49 (MQ=255)
                                                                               ttGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATg                                 >  1:194911/1‑139 (MQ=255)
                                                                                tGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGAc                                                                                                                           <  1:796783/48‑1 (MQ=255)
                                                                                tGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGAc                                                                                                                           >  2:796783/1‑48 (MQ=255)
                                                                                tGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                >  2:510128/1‑139 (MQ=255)
                                                                                    ttCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                                      >  1:1339433/1‑65 (MQ=255)
                                                                                    ttCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                                      <  2:1339433/65‑1 (MQ=255)
                                                                                    ttCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            <  1:510128/139‑1 (MQ=255)
                                                                                     tCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa                           <  2:719164/139‑1 (MQ=255)
                                                                                     tCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa                           <  2:27316/139‑1 (MQ=255)
                                                                                      cAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGagag                             <  1:727813/136‑1 (MQ=255)
                                                                                      cAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGagag                             >  2:727813/1‑136 (MQ=255)
                                                                                      cAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCat                          >  2:1190791/1‑139 (MQ=255)
                                                                                       aGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTc                                                                                                           <  1:1014230/57‑1 (MQ=255)
                                                                                       aGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTc                                                                                                           >  2:1014230/1‑57 (MQ=255)
                                                                                       aGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCata                         >  2:643896/1‑139 (MQ=255)
                                                                                         cGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            >  1:513874/1‑134 (MQ=255)
                                                                                         cGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            <  2:513874/134‑1 (MQ=255)
                                                                                            agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                                      >  2:1212165/1‑57 (MQ=255)
                                                                                            agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGa                                                                                                      <  1:1212165/57‑1 (MQ=255)
                                                                                            agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                              <  2:934679/113‑1 (MQ=255)
                                                                                            agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                              >  1:934679/1‑113 (MQ=255)
                                                                                            agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt                    <  1:643896/139‑1 (MQ=255)
                                                                                                aCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                <  2:582124/123‑1 (MQ=255)
                                                                                                aCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                >  1:582124/1‑123 (MQ=255)
                                                                                                 cGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGAt               <  2:1279350/139‑1 (MQ=255)
                                                                                                     cccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                              <  2:1007980/104‑1 (MQ=255)
                                                                                                     cccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                              >  1:1007980/1‑104 (MQ=255)
                                                                                                            tGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGTCTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGTCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCgaga    <  1:900898/139‑1 (MQ=255)
                                                                                                              aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            >  2:68755/1‑113 (MQ=255)
                                                                                                              aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            <  1:68755/113‑1 (MQ=255)
                                                                                                              aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGaaa  >  1:147414/1‑139 (MQ=255)
                                                                                                                  |                                                                                                                                      
ACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAA  >  NC_000913/4511032‑4511280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A