Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,690	C→T	256 (TAG→TAA)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,690	0	C	T	100.0%	177.3 / NA	55	256 (TAG→TAA)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/34); total (21/34)

GCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGTTT  >  NC_000913/4510554‑4510822

gCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTa                                                                                                                                    <  1:748152/139‑1 (MQ=255)

           gcGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGc                                                                                                                         >  1:663922/1‑139 (MQ=255)

                tGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATc                                                                                                                    >  2:258269/1‑139 (MQ=255)

                tGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATc                                                                                                                    >  2:310686/1‑139 (MQ=255)

                 ggACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCg                                                                                                                   <  1:56728/139‑1 (MQ=255)

                   aCGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  2:359885/139‑1 (MQ=255)

                   aCGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  2:33810/139‑1 (MQ=255)

                             ttGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                       <  2:621609/139‑1 (MQ=255)

                             ttGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                       <  1:791175/139‑1 (MQ=255)

                              tGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATAc                                                                                                      <  1:191918/139‑1 (MQ=255)

                                   aTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATAcc                                                                                                     >  2:731007/1‑135 (MQ=255)

                                   aTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATAcc                                                                                                     <  1:731007/135‑1 (MQ=255)

                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               >  1:465607/1‑139 (MQ=255)

                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               <  1:788017/139‑1 (MQ=255)

                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               >  2:576707/1‑139 (MQ=255)

                                       ttCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCggg                                                                                             <  2:703818/139‑1 (MQ=255)

                                       ttCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCggg                                                                                             <  1:576707/139‑1 (MQ=255)

                                         cAGAGTCCGGAAAAGTTCAGCCCCTGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGgt                                                                                           <  1:616868/139‑1 (MQ=255)

                                               ccGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATGCCGGCTCGGGGTGTAttt                                                                                     >  1:709688/1‑139 (MQ=255)

                                                cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTc                                                                                    >  1:463864/1‑139 (MQ=255)

                                                cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTc                                                                                    <  1:1053114/139‑1 (MQ=255)

                                                cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGACGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCGCGGGGTGTATTTc                                                                                    >  2:1053114/1‑139 (MQ=255)

                                                       gTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTcc                                                                             <  1:795507/139‑1 (MQ=255)

                                                        ttCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTcc                                                                             >  2:271664/1‑138 (MQ=255)

                                                        ttCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTcc                                                                             <  1:271664/138‑1 (MQ=255)

                                                                       aaCAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTtc                                                             >  2:868313/1‑139 (MQ=255)

                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGTTTCTTCCGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  1:973575/139‑1 (MQ=255)

                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:554312/139‑1 (MQ=255)

                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:463864/139‑1 (MQ=255)

                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:709688/139‑1 (MQ=255)

                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  1:258269/139‑1 (MQ=255)

                                                                            gCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGc                                                        >  2:739236/1‑139 (MQ=255)

                                                                             cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 >  1:874728/1‑81 (MQ=255)

                                                                             cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  2:874728/81‑1 (MQ=255)

                                                                                 cTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATcc                                                   >  2:57701/1‑139 (MQ=255)

                                                                                           tccagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGtcatc                                         <  1:979979/139‑1 (MQ=255)

                                                                                           tccagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGtcatc                                         <  1:57701/139‑1 (MQ=255)

                                                                                             cagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGTGGTGTATTTCCGCGTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCAc                                       <  1:195857/139‑1 (MQ=255)

                                                                                                tttCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCTGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACctc                                    <  1:865329/139‑1 (MQ=255)

                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACAt                                                                                                                     >  2:879510/1‑53 (MQ=255)

                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACAt                                                                                                                     <  1:879510/53‑1 (MQ=255)

                                                                                                             tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCt                       <  1:739236/139‑1 (MQ=255)

                                                                                                                    gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:44115/98‑1 (MQ=255)

                                                                                                                    gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         >  1:44115/1‑98 (MQ=255)

                                                                                                                    gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCa                <  2:465607/139‑1 (MQ=255)

                                                                                                                    gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCa                >  1:126958/1‑139 (MQ=255)

                                                                                                                          aCGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                                 <  2:654831/36‑1 (MQ=255)

                                                                                                                               cTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCg     <  1:310686/139‑1 (MQ=255)

                                                                                                                                tgtgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCAACACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGt    <  2:522441/139‑1 (MQ=255)

                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACTGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGttt  >  1:703901/1‑139 (MQ=255)

                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCaa                                                         <  2:595315/84‑2 (MQ=255)

                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCaa                                                         >  1:595315/1‑83 (MQ=255)

                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTg                     <  2:670800/120‑1 (MQ=255)

                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTg                     >  1:670800/1‑120 (MQ=255)

                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGttt  >  2:147542/1‑139 (MQ=255)

GCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGTTT  >  NC_000913/4510554‑4510822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A