Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I1 R1
|
759 |
71.0 |
3673240 |
91.5% |
3361014 |
104.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,399,985 |
(C)7→8 |
coding (252/516 nt) |
ogt ← |
O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.4%
| 129.7
/ ‑3.2
| 38 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (18/19); total (18/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 |
GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399841‑1400111
|
gggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑c < 2:14298/139‑1 (MQ=255)
aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc < 2:514881/139‑1 (MQ=255)
tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc > 1:314258/1‑139 (MQ=255)
cGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGTCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑c < 2:441661/139‑1 (MQ=255)
cGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑c < 1:1167841/139‑1 (MQ=255)
aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccg < 1:1294919/139‑3 (MQ=255)
aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccg < 2:314258/139‑3 (MQ=255)
cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccgtag < 1:742513/139‑6 (MQ=255)
gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGc > 1:1618950/1‑139 (MQ=255)
tGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccgtag > 2:76574/1‑130 (MQ=255)
tGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccgtag < 1:76574/135‑6 (MQ=255)
gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGa < 1:1135659/139‑1 (MQ=255)
aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAg < 1:1403992/139‑1 (MQ=255)
aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCGCGTAGCAGTGGGAAg < 1:1226323/139‑1 (MQ=255)
ggCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGc < 1:799068/124‑1 (MQ=255)
ggCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGc > 2:799068/1‑124 (MQ=255)
gACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTAc > 1:422658/1‑139 (MQ=255)
cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 2:867498/1‑139 (MQ=255)
cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 2:1831195/1‑139 (MQ=255)
aaTTGCTCAACCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCaaaa < 2:544185/139‑1 (MQ=255)
ttGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata < 2:1618950/139‑1 (MQ=255)
cAGCCAGTTGGCCGCAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCCGGGGGAAGCGTATCCATAAAGCTAAGATTACCGGCCAAATATTCAc > 1:381632/1‑139 (MQ=255)
gTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGGAGCGTATCAATAATGATAAGATTACCGGCAAAATATTCACGAAGCTTGTCGcc > 2:852932/1‑138 (MQ=255)
tAATGCATTATCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCT‑AGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTa < 1:1581439/139‑1 (MQ=255)
aaTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCt > 1:461473/1‑101 (MQ=255)
aaTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCt < 2:461473/101‑1 (MQ=255)
aaTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTa < 2:422658/139‑1 (MQ=255)
aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:1671165/139‑1 (MQ=255)
aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 2:571414/139‑1 (MQ=255)
cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCaat < 2:1818754/82‑1 (MQ=255)
cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCaat > 1:1818754/1‑82 (MQ=255)
ggggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACTGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGc > 1:475133/1‑139 (MQ=255)
gATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAGGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGa < 1:867498/139‑1 (MQ=255)
gTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccgtag > 2:193504/1‑45 (MQ=255)
gTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc‑ccgtag < 1:193504/50‑6 (MQ=255)
gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCaa < 1:1623042/66‑1 (MQ=255)
gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCaa > 2:1623042/1‑66 (MQ=255)
gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcg > 1:1729727/1‑139 (MQ=255)
cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 2:31608/139‑1 (MQ=255)
tttCCAGACTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGcc > 1:1074045/1‑139 (MQ=255)
aCTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCAt < 2:887464/128‑1 (MQ=255)
aCTTCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCAt > 1:887464/1‑128 (MQ=255)
ttCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 2:607511/1‑85 (MQ=255)
ttCGCGCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 1:607511/85‑1 (MQ=255)
cgcgCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTAt > 2:675279/1‑43 (MQ=255)
cgcgCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTAt < 1:675279/43‑1 (MQ=255)
cgcgCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGcc < 2:1832226/98‑1 (MQ=255)
cgcgCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGcc > 1:1832226/1‑98 (MQ=255)
cgcgCTGAAATGGCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 1:597620/1‑139 (MQ=255)
ggCGTCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:1831195/139‑1 (MQ=255)
|
GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399841‑1400111
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
