Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
757 |
76.1 |
3598070 |
93.4% |
3360597 |
108.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,399,985 |
(C)7→8 |
coding (252/516 nt) |
ogt ← |
O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.9%
| 164.8
/ ‑3.4
| 48 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (21/26); total (22/26) |
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 |
ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111
|
aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccc > 2:996197/1‑139 (MQ=255)
aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccc < 1:863679/139‑1 (MQ=255)
ttCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc < 1:996197/139‑1 (MQ=255)
aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc‑cccg < 1:1294309/139‑3 (MQ=255)
cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc‑cccgtag < 2:705655/139‑6 (MQ=255)
cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCCCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc‑cccgtag < 1:1536610/139‑6 (MQ=255)
gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGc < 2:10197/139‑1 (MQ=255)
cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTggg > 1:300217/1‑139 (MQ=255)
gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGa < 2:300217/139‑1 (MQ=255)
cACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGaa < 1:96322/139‑1 (MQ=255)
aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAg < 2:212936/139‑1 (MQ=255)
cGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTAt < 2:193996/139‑1 (MQ=255)
gCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCATTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCa < 1:799123/139‑1 (MQ=255)
aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaat < 1:524382/139‑1 (MQ=255)
gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGt > 1:1514622/1‑131 (MQ=255)
gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGt < 2:1514622/131‑1 (MQ=255)
cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaata > 2:1455789/1‑137 (MQ=255)
cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaata < 1:1455789/137‑1 (MQ=255)
gCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGc < 2:1719792/139‑1 (MQ=255)
cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 2:1326493/1‑139 (MQ=255)
cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa < 1:358763/139‑1 (MQ=255)
cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCa > 2:868903/1‑93 (MQ=255)
cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCa < 1:868903/93‑1 (MQ=255)
tGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTg > 2:1072759/1‑139 (MQ=255)
tgtaTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaaa < 2:891302/136‑1 (MQ=255)
aCCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCt < 1:417292/139‑1 (MQ=255)
gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata > 2:157596/1‑107 (MQ=255)
gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata < 1:157596/107‑1 (MQ=255)
gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 2:308987/1‑139 (MQ=255)
gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 1:1301906/1‑139 (MQ=255)
gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 2:1315817/1‑139 (MQ=255)
cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAg < 2:319691/73‑1 (MQ=255)
cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAg > 1:319691/1‑73 (MQ=255)
ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtg > 2:463753/1‑139 (MQ=255)
gggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCa < 1:1697825/139‑1 (MQ=255)
tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGa > 2:1728136/1‑57 (MQ=255)
tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGa < 1:1728136/57‑1 (MQ=255)
gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 2:1385754/1‑103 (MQ=255)
gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 1:1385754/103‑1 (MQ=255)
cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 1:308987/139‑1 (MQ=255)
aCTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTg > 1:1300962/1‑139 (MQ=255)
cgcgCTGAAATGGCGTCCCCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGACTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGa > 1:32811/1‑139 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaa < 1:1063425/46‑1 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaa > 2:1063425/1‑46 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg < 2:973098/96‑1 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg > 1:973098/1‑96 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc > 2:249342/1‑118 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc < 1:249342/118‑1 (MQ=255)
cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 2:1526833/1‑139 (MQ=255)
cacgcTGAAATGGCGT‑‑CCCCCTCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTGAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 1:1079211/3‑139 (MQ=255)
gcgcTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 2:1501196/82‑1 (MQ=255)
gcgcTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 1:1501196/1‑82 (MQ=255)
cgcTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATaa < 1:1326493/139‑1 (MQ=255)
ggCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:463753/139‑1 (MQ=255)
|
ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
