Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,399,985 |
(C)7→8 |
coding (252/516 nt) |
ogt ← |
O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,399,978 | 1 | . | C | 100.0%
| 54.9
/ NA
| 17 | T87A (ACG→GCG) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (9/8); total (9/8) |
GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAT > NC_000913/1399842‑1400100
|
ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cc < 2:16754/139‑1 (MQ=255)
aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cccc > 2:186953/1‑139 (MQ=255)
tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cccccc > 1:97515/1‑139 (MQ=255)
tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cccccc < 1:632574/139‑1 (MQ=255)
cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTTCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cccccccccgta < 2:379310/139‑6 (MQ=255)
gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑ccccccccgta > 2:254028/1‑133 (MQ=255)
gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑ccccccccgta < 1:254028/137‑5 (MQ=255)
aaCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGt < 1:536829/139‑1 (MQ=255)
gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTCCCCCCCCCGTAGCAGTGGGAGGCGTATCaata > 1:216377/1‑139 (MQ=255)
cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCaataat > 1:259545/1‑139 (MQ=255)
cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGc > 1:123532/1‑102 (MQ=255)
cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGc < 2:123532/102‑1 (MQ=255)
ttGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata < 2:259545/139‑1 (MQ=255)
cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:401309/1‑139 (MQ=255)
cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 2:508697/1‑139 (MQ=255)
ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAACTGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtg > 2:711599/1‑139 (MQ=255)
cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATTTTCACGAAGCTTGTCGCTTAAACCGCCCGGATtgg > 1:460029/1‑139 (MQ=255)
tgttggttgtgtTTTCCAGACATCCCGCCTGAATTGGCGC‑‑CCCACACGTAGCAGTGGGAAGCGTATCAATAATGCTAAGAGTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAAt < 2:97515/131‑1 (MQ=255)
gTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:374666/139‑1 (MQ=255)
tGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAg < 1:706036/59‑1 (MQ=255)
tGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAg > 2:706036/1‑59 (MQ=255)
cGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cccc > 1:432520/1‑39 (MQ=255)
cGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑cccc < 2:432520/39‑1 (MQ=255)
gTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCt > 1:255459/1‑139 (MQ=255)
tAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTc < 1:295396/139‑1 (MQ=255)
ttCGCG‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCg < 2:401309/139‑1 (MQ=255)
cgcg‑CTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt < 1:186953/139‑1 (MQ=255)
|
GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCG‑CTGAAATGGCGT‑‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAT > NC_000913/1399842‑1400100
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
