Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	821,506	G→A	238 (TAG→TAA)	ybhM →	BAX Inhibitor‑1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	821,506	0	G	A	100.0%	190.9 / NA	59	238 (TAG→TAA)	ybhM	BAX Inhibitor‑1 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/30); total (29/30)

TTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGAT  >  NC_000913/821375‑821628

ttGTGGAGTCCGATTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTaaa                                                                                                                     >  2:745497/1‑139 (MQ=255)

   tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATA‑CTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 <  1:1505186/139‑1 (MQ=255)

    ggAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 <  1:1208567/139‑1 (MQ=255)

    ggAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 <  2:337395/139‑1 (MQ=255)

    ggAGTCCGATTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 <  1:745497/139‑1 (MQ=255)

     gAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcac                                                                                                                >  1:794807/1‑139 (MQ=255)

               cGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                       <  1:64949/122‑1 (MQ=255)

               cGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                       >  2:64949/1‑122 (MQ=255)

                      cTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                >  1:585549/1‑138 (MQ=255)

                      cTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                <  2:585549/138‑1 (MQ=255)

                                          ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 >  2:1136559/1‑101 (MQ=255)

                                          ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 <  1:1136559/101‑1 (MQ=255)

                                          ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCtt                                                                           >  2:300831/1‑139 (MQ=255)

                                             tATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCAt                                                                        >  2:1028583/1‑139 (MQ=255)

                                               ttACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTaaa                                                                                           <  2:1179345/118‑1 (MQ=255)

                                               ttACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTaaa                                                                                           >  1:1179345/1‑118 (MQ=255)

                                                     ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCCTTAACCGTAAATGCACATACTAAAg                                                                                                       >  2:1114393/1‑100 (MQ=255)

                                                     ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAg                                                                                                       <  1:1114393/100‑1 (MQ=255)

                                                     ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                <  1:1028583/139‑1 (MQ=255)

                                                         aTCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTaaa                                                                                           >  2:663836/1‑108 (MQ=255)

                                                         aTCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTaaa                                                                                           <  1:663836/108‑1 (MQ=255)

                                                             gCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGctt                                                        >  2:150042/1‑139 (MQ=255)

                                                               ttcttTGGTATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGcttct                                                      >  2:1476538/1‑139 (MQ=255)

                                                                     gggATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCt                                                <  1:1476538/139‑1 (MQ=255)

                                                                           cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                       <  2:431384/62‑1 (MQ=255)

                                                                           cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                       >  1:431384/1‑62 (MQ=255)

                                                                           cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                >  1:291065/1‑117 (MQ=255)

                                                                           cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                <  2:291065/117‑1 (MQ=255)

                                                                            ttGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAAc                                                                                               <  1:777878/85‑1 (MQ=255)

                                                                            ttGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAAc                                                                                               >  2:777878/1‑85 (MQ=255)

                                                                              gCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCa                                       >  1:1298456/1‑139 (MQ=255)

                                                                                cgcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAg                                      >  1:1462956/1‑138 (MQ=255)

                                                                                cgcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAg                                      <  2:1462956/138‑1 (MQ=255)

                                                                                cgcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGc                                     >  1:861940/1‑139 (MQ=255)

                                                                                 gcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCa                                    <  1:150042/139‑1 (MQ=255)

                                                                                  cgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                   <  1:715417/139‑1 (MQ=255)

                                                                                  cgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                   <  1:300831/139‑1 (MQ=255)

                                                                                              cGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 >  2:87227/1‑49 (MQ=255)

                                                                                              cGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                 <  1:87227/49‑1 (MQ=255)

                                                                                                 ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAg                    >  2:952047/1‑139 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACAt                                                                                                              <  2:681763/48‑1 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACAt                                                                                                              >  1:681763/1‑48 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                   >  1:186291/1‑123 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                   <  2:186291/123‑1 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCa                                    >  2:491948/1‑122 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCa                                    <  1:491948/122‑1 (MQ=255)

                                                                                                  tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAgc                   <  2:1298456/139‑1 (MQ=255)

                                                                                                   gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAAt                                                                                                                    >  1:1503187/1‑41 (MQ=255)

                                                                                                   gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAAt                                                                                                                    <  2:1503187/41‑1 (MQ=255)

                                                                                                   gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                               <  2:1375530/46‑1 (MQ=255)

                                                                                                   gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                               >  1:1375530/1‑46 (MQ=255)

                                                                                                        gCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagc             <  2:1141365/139‑1 (MQ=255)

                                                                                                        gCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagc             >  1:1141365/1‑139 (MQ=255)

                                                                                                          aaaGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagcag           <  2:861940/139‑1 (MQ=255)

                                                                                                                   cgcgTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGAt  >  2:158945/1‑139 (MQ=255)

                                                                                                                        ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCa                     <  2:476246/115‑1 (MQ=255)

                                                                                                                        ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCa                     >  1:476246/1‑115 (MQ=255)

                                                                                                                        ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagcagcag        >  1:1503875/1‑128 (MQ=255)

                                                                                                                        ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagcagcag        <  2:1503875/128‑1 (MQ=255)

TTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGAT  >  NC_000913/821375‑821628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A