Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	821,506	G→A	238 (TAG→TAA)	ybhM →	BAX Inhibitor‑1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	821,506	0	G	A	100.0%	163.2 / NA	52	238 (TAG→TAA)	ybhM	BAX Inhibitor‑1 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/25); total (27/25)

TGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATA  >  NC_000913/821378‑821629

tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  1:606465/139‑1 (MQ=255)

 ggAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  2:771745/139‑1 (MQ=255)

            cGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCg                                                                                                                          >  2:448315/1‑120 (MQ=255)

            cGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCg                                                                                                                          <  1:448315/120‑1 (MQ=255)

                     tttATAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTa                                                                                              <  1:702929/139‑1 (MQ=255)

                                       ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   >  2:391294/1‑100 (MQ=255)

                                       ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  1:391294/100‑1 (MQ=255)

                                            ttACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCAttt                                                                       >  1:315972/1‑139 (MQ=255)

                                                  ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        >  1:431911/1‑84 (MQ=255)

                                                  ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        <  2:431911/84‑1 (MQ=255)

                                                  ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   >  2:251580/1‑89 (MQ=255)

                                                  ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  1:251580/89‑1 (MQ=255)

                                                  ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                 >  1:432464/1‑139 (MQ=255)

                                                    ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGTCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACtt                                                                                                   >  2:80416/1‑103 (MQ=255)

                                                    ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACtt                                                                                                   <  1:80416/103‑1 (MQ=255)

                                                    ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCg                                                               >  2:215357/1‑139 (MQ=255)

                                                    ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCg                                                               >  1:280337/1‑139 (MQ=255)

                                                    ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCg                                                               >  1:725724/1‑139 (MQ=255)

                                                      aTCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   >  2:214614/1‑85 (MQ=255)

                                                      aTCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  1:214614/85‑1 (MQ=255)

                                                      aTCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTa                                                             >  1:800068/1‑139 (MQ=255)

                                                          gCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTTTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGctt                                                         >  2:198045/1‑139 (MQ=255)

                                                            ttcttTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGcttct                                                       >  2:518855/1‑139 (MQ=255)

                                                                       aCTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCtt                                            <  2:256173/139‑1 (MQ=255)

                                                                         ttGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTc                                          >  2:722924/1‑139 (MQ=255)

                                                                             cgcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGc                                      >  1:31468/1‑139 (MQ=255)

                                                                               cgATAGCTATAACGCTTTTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                    <  1:198045/139‑1 (MQ=255)

                                                                                 aTAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  <  1:722924/139‑1 (MQ=255)

                                                                                 aTAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  <  2:31468/139‑1 (MQ=255)

                                                                                     cTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTa                              >  2:597252/1‑139 (MQ=255)

                                                                                          aCGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGc                                      >  2:369225/1‑126 (MQ=255)

                                                                                          aCGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGc                                      <  1:369225/126‑1 (MQ=255)

                                                                                              ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  <  2:317085/126‑1 (MQ=255)

                                                                                              ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  >  1:317085/1‑126 (MQ=255)

                                                                                                gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        <  2:164793/38‑1 (MQ=255)

                                                                                                gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        >  1:164793/1‑38 (MQ=255)

                                                                                                gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAgcg                   >  1:61020/1‑139 (MQ=255)

                                                                                                     gCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagc              <  2:315972/139‑1 (MQ=255)

                                                                                                          ggCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagcagcag         <  2:432464/139‑1 (MQ=255)

                                                                                                             aTACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  <  2:376408/111‑1 (MQ=255)

                                                                                                             aTACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  >  1:376408/1‑111 (MQ=255)

                                                                                                             aTACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTgg      <  1:215357/139‑1 (MQ=255)

                                                                                                               aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    <  1:597252/139‑1 (MQ=255)

                                                                                                               aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    <  2:188488/139‑1 (MQ=255)

                                                                                                               aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    >  2:534782/1‑139 (MQ=255)

                                                                                                               aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    >  1:556700/1‑139 (MQ=255)

                                                                                                               aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    <  2:730322/139‑1 (MQ=255)

                                                                                                               aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    >  1:188488/1‑139 (MQ=255)

                                                                                                                cgcgTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGAt   >  1:51988/1‑139 (MQ=255)

                                                                                                                 gcgTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATa  <  1:534782/139‑1 (MQ=255)

                                                                                                                     ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    >  2:588257/1‑133 (MQ=255)

                                                                                                                     ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa    <  1:588257/133‑1 (MQ=255)

TGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATA  >  NC_000913/821378‑821629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A